Complete inhibition of a polyol nucleation by a micromolar biopolymer additive.
Journal
Cell reports. Physical science
ISSN: 2666-3864
Titre abrégé: Cell Rep Phys Sci
Pays: United States
ID NLM: 101769239
Informations de publication
Date de publication:
16 Feb 2022
16 Feb 2022
Historique:
entrez:
10
3
2022
pubmed:
11
3
2022
medline:
11
3
2022
Statut:
ppublish
Résumé
Preventing spontaneous crystallization of supersaturated solutions by additives is of critical interest to successful process design and implementation, with numerous applications in chemical, pharmaceutical, medical, pigment, and food industries, but challenges remain in laboratory and industry settings and fundamental understanding is lacking. When copresented with antifreeze proteins (AFPs), otherwise spontaneously crystallizing osmolytes are maintained at high supersaturations for months in over-wintering organisms. Thus, we here explore the inhibition phenomenon by AFPs, using persistent crystallization of a common sugar alcohol, D-mannitol, as a case study. We report experimentally that DAFP1, an insect AFP, completely inhibits D-mannitol nucleation. Computer simulations reveal a new mechanism for crystallization inhibition where the population of the crystal-forming conformers are selectively bound and randomized in solution by hydrogen bonding to the protein surface. These results highlight the advantages of using natural polymers to address crystallization inhibition challenges and suggest new strategies in controlling the nucleation processes.
Identifiants
pubmed: 35265868
doi: 10.1016/j.xcrp.2021.100723
pmc: PMC8903182
mid: NIHMS1781668
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NCRR NIH HHS
ID : S10 RR025631
Pays : United States
Organisme : NIGMS NIH HHS
ID : SC3 GM086249
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM008228
Pays : United States
Déclaration de conflit d'intérêts
DECLARATION OF INTERESTS The authors declare no competing interests.
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