RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
RACK1
RNA-binding proteins
SERBP1
Vigilin
dengue virus
host factors
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
13 04 2022
13 04 2022
Historique:
pubmed:
11
3
2022
medline:
16
4
2022
entrez:
10
3
2022
Statut:
ppublish
Résumé
Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no effective treatment is available. DENV relies heavily on the host cellular machinery for productive infection. Here, we show that the scaffold protein RACK1, which is part of the DENV replication complex, mediates infection by binding to the 40S ribosomal subunit. Mass spectrometry analysis of RACK1 partners coupled to an RNA interference screen-identified Vigilin and SERBP1 as DENV host-dependency factors. Both are RNA-binding proteins that interact with the DENV genome. Genetic ablation of Vigilin or SERBP1 rendered cells poorly susceptible to DENV, as well as related flaviviruses, by hampering the translation and replication steps. Finally, we established that a Vigilin or SERBP1 mutant lacking RACK1 binding but still interacting with the viral RNA is unable to mediate DENV infection. We propose that RACK1 recruits Vigilin and SERBP1, linking the DENV genome to the translation machinery for efficient infection.
Identifiants
pubmed: 35266803
doi: 10.1128/jvi.01962-21
pmc: PMC9006918
doi:
Substances chimiques
Neoplasm Proteins
0
RACK1 protein, human
0
RNA, Viral
0
RNA-Binding Proteins
0
Receptors for Activated C Kinase
0
SERBP1 protein, human
0
high density lipoprotein binding protein
147605-06-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0196221Références
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