Quantification of Bacterial DNA from Infected Human Root Canals Using qPCR and DAPI after Disinfection with Established and Novel Irrigation Protocols.
endodontics
irrigation protocol
qPCR–DAPI
root canal system
silver diamine fluoride
Journal
Materials (Basel, Switzerland)
ISSN: 1996-1944
Titre abrégé: Materials (Basel)
Pays: Switzerland
ID NLM: 101555929
Informations de publication
Date de publication:
04 Mar 2022
04 Mar 2022
Historique:
received:
31
01
2022
revised:
23
02
2022
accepted:
25
02
2022
entrez:
10
3
2022
pubmed:
11
3
2022
medline:
11
3
2022
Statut:
epublish
Résumé
The removal of bacterial infections within the root canal system is still a challenge. Therefore, the cleansing effect of established and new irrigation-protocols (IP) containing silver diamine fluoride (SDF) 3.8% on the whole root canal system was analyzed using quantitative PCR (qPCR) and 4',6-diamidino-phenylindole-(DAPI)-staining. Extracted human premolars were instrumented up to F2 (ProTaper Gold) under NaCl 0.9% irrigation and incubated with
Identifiants
pubmed: 35269141
pii: ma15051911
doi: 10.3390/ma15051911
pmc: PMC8912041
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : WE5838/1-1, DA 1701/2-1 and WE 5838/1-2, DA 1701/2-1
Références
J Endod. 2015 Aug;41(8):1226-33
pubmed: 25906920
Microbiologyopen. 2017 Aug;6(4):
pubmed: 28244230
J Endod. 1990 Dec;16(12):580-8
pubmed: 2094761
J Endod. 2015 Apr;41(4):447-50
pubmed: 25576208
J Clin Microbiol. 2008 Nov;46(11):3599-606
pubmed: 18768651
Braz Dent J. 2016 Jan-Feb;27(1):3-8
pubmed: 27007337
Curr Microbiol. 2020 Apr;77(4):682-687
pubmed: 31811375
Eur Arch Paediatr Dent. 2021 Jun;22(3):515-525
pubmed: 32677019
Antimicrob Resist Infect Control. 2019 Jan 17;8:16
pubmed: 30675343
Int Endod J. 2010 Mar;43(3):241-50
pubmed: 20158536
Int Endod J. 2017 Jan;50(1):77-83
pubmed: 26660494
J Dent Res. 2019 Jan;98(1):14-26
pubmed: 30290130
Oral Microbiol Immunol. 2008 Dec;23(6):449-54
pubmed: 18954349
Int Endod J. 2001 Jul;34(5):399-405
pubmed: 11482724
Clin Exp Dent Res. 2019 Jul 16;5(5):551-556
pubmed: 31687190
Clin Oral Investig. 2019 Dec;23(12):4325-4334
pubmed: 30888514
J Endod. 2010 Jun;36(6):1026-9
pubmed: 20478459
J Dent Res. 2017 Jun;96(6):626-632
pubmed: 28530469
Contemp Clin Dent. 2012 Jul;3(3):262-4
pubmed: 23293478
Arch Oral Biol. 2005 Jun;50(6):575-83
pubmed: 15848151
Acta Odontol Scand. 2021 May;79(4):302-308
pubmed: 33306916
Caries Res. 2013;47(4):284-90
pubmed: 23392087
Arch Oral Biol. 2017 Nov;83:289-296
pubmed: 28865352
Crit Rev Oral Biol Med. 2002;13(2):171-83
pubmed: 12097359
Acta Odontol Scand. 2019 Jan;77(1):55-60
pubmed: 30264624
Clin Oral Investig. 2018 Mar;22(2):655-670
pubmed: 29372445
Clin Oral Investig. 2018 Jan;22(1):267-274
pubmed: 28349219
Front Microbiol. 2016 Nov 22;7:1833
pubmed: 27920757
J Endod. 1996 Dec;22(12):674-6
pubmed: 9220753
J Endod. 2008 Nov;34(11):1291-1301.e3
pubmed: 18928835
Antibiotics (Basel). 2021 Dec 19;10(12):
pubmed: 34943764
Int Endod J. 2017 Oct;50(10):976-982
pubmed: 27809342
Clin Oral Investig. 2017 Dec;21(9):2681-2687
pubmed: 28185091
J Endod. 2019 Jan;45(1):31-44.e13
pubmed: 30558797
J Dent. 2014 Aug;42(8):945-51
pubmed: 24930870
J Clin Diagn Res. 2016 Jun;10(6):ZC71-7
pubmed: 27504415
J Endod. 2004 May;30(5):315-20
pubmed: 15107642
Front Genet. 2020 Dec 18;11:599137
pubmed: 33391351
J Dent. 2012 Jul;40(7):531-41
pubmed: 22484380
Int Endod J. 1998 Jan;31(1):1-7
pubmed: 9823122
J Endod. 2001 Jan;27(1):1-6
pubmed: 11487156
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004 Jan;97(1):85-94
pubmed: 14716262