FT-IR Spectral Signature of Sensitive and Multidrug-Resistant Osteosarcoma Cell-Derived Extracellular Nanovesicles.
FT-IR/ATR spectroscopy
extracellular nanovesicles
multidrug resistance
non-invasive diagnostics
osteosarcoma
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
23 02 2022
23 02 2022
Historique:
received:
01
02
2022
revised:
16
02
2022
accepted:
21
02
2022
entrez:
10
3
2022
pubmed:
11
3
2022
medline:
12
4
2022
Statut:
epublish
Résumé
Osteosarcoma (OS) is the most common primary bone cancer in children and adolescents. Despite aggressive treatment regimens, the outcome is unsatisfactory, and multidrug resistance (MDR) is a pivotal process in OS treatment failure. OS-derived extracellular vesicles (EVs) promote drug resistance to chemotherapy and target therapy through different mechanisms. The aim of this study was to identify subpopulations of osteosarcoma-EVs by Fourier transform infrared spectroscopy (FT-IR) to define a specific spectral signature for sensitive and multidrug-resistant OS-derived EVs. EVs were isolated from sensitive and MDR OS cells as well as from mesenchymal stem cells by differential centrifugation and ultracentrifugation. EVs size, morphology and protein expression were characterized. FT-IR/ATR of EVs spectra were acquired in the region of 400-4000 cm
Identifiants
pubmed: 35269400
pii: cells11050778
doi: 10.3390/cells11050778
pmc: PMC8909163
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Italian Ministry of Health
ID : 5xMille 2020
Organisme : AIRC IG 2018
ID : Project Number 21403
Organisme : Italian Ministry of Health
ID : SG-2018-12367059
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