Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization.
cytokines
exosome markers
growth factors
human platelets
platelet-derived-exosomes
platelet-rich plasma
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
05 Mar 2022
05 Mar 2022
Historique:
received:
31
01
2022
revised:
25
02
2022
accepted:
04
03
2022
entrez:
10
3
2022
pubmed:
11
3
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Platelet-Rich Plasma (PRP) is enriched in molecular messengers with restorative effects on altered tissue environments. Upon activation, platelets release a plethora of growth factors and cytokines, either in free form or encapsulated in exosomes, which have been proven to promote tissue repair and regeneration. Translational research on the potential of exosomes as a safe nanosystem for therapeutic cargo delivery requires standardizing exosome isolation methods along with their molecular and morphological characterization. With this aim, we isolated and characterized the exosomes released by human PRP platelets. Western blot analysis revealed that CaCl
Identifiants
pubmed: 35270001
pii: ijms23052861
doi: 10.3390/ijms23052861
pmc: PMC8911307
pii:
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Basque Government
ID : IT1230-19
Organisme : AlavaInnova
Organisme : Ministry of Economy, Industry and Competitiveness
ID : CTQ2017-85686-R
Organisme : Centro de Investigación Biomédica en Red de Salud Mental
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