Protease-activated receptor 2 enhances innate and inflammatory mechanisms induced by lipopolysaccharide in macrophages from C57BL/6 mice.
Animals
Cytokines
/ metabolism
Lipopolysaccharides
/ pharmacology
Macrophages
Mice
Mice, Inbred C57BL
NF-kappa B
/ metabolism
Nitric Oxide
/ metabolism
Nitric Oxide Synthase Type II
/ metabolism
Reactive Oxygen Species
/ metabolism
Receptor, PAR-2
/ metabolism
Tumor Necrosis Factor-alpha
/ metabolism
LPS
Macrophages
PAR2
Journal
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN: 1420-908X
Titre abrégé: Inflamm Res
Pays: Switzerland
ID NLM: 9508160
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
25
05
2021
accepted:
16
02
2022
revised:
11
02
2022
pubmed:
12
3
2022
medline:
12
4
2022
entrez:
11
3
2022
Statut:
ppublish
Résumé
This study was conducted to investigate the effects of the synthetic PAR2 agonist peptide (PAR2-AP) SLIGRL-NH Peritoneal macrophages obtained from C57BL/6 mice were incubated with PAR2-AP and/or LPS, and the phagocytosis of zymosan fluorescein isothiocyanate (FITC) particles; nitric oxide (NO), reactive oxygen species (ROS), and cytokine production; and inducible NO synthase (iNOS) expression in macrophages co-cultured with PAR-2-AP/LPS were evaluated. Co-incubation of macrophages with PAR2AP (30 µM)/LPS (100 ng/mL) enhanced LPS-induced phagocytosis; production of NO, ROS, and the pro-inflammatory cytokines interleukin (IL)-1β, tumour necrosis factor (TNF)-α, IL-6, and C-C motif chemokine ligand (CCL)2; and iNOS expression and impaired the release of the anti-inflammatory cytokine IL-10 after 4 h of co-stimulation. In addition, PAR2AP increased the LPS-induced translocation of the p65 subunit of the pro-inflammatory transcription factor nuclear factor kappa B (NF-κB) and reduced the expression of inhibitor of NF-κB. This study provides evidence of a role for PAR2 in macrophage response triggered by LPS enhancing the phagocytic activity and NO, ROS, and cytokine production, resulting in the initial and adequate macrophage response required for their innate response mechanisms.
Identifiants
pubmed: 35274151
doi: 10.1007/s00011-022-01551-9
pii: 10.1007/s00011-022-01551-9
doi:
Substances chimiques
Cytokines
0
F2rl1 protein, mouse
0
Lipopolysaccharides
0
NF-kappa B
0
Reactive Oxygen Species
0
Receptor, PAR-2
0
Tumor Necrosis Factor-alpha
0
Nitric Oxide
31C4KY9ESH
Nitric Oxide Synthase Type II
EC 1.14.13.39
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
439-448Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : PPM-00593-16
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
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