Sperm-oviduct interaction: Differential gene expression of growth factors induced by sperm DNA fragmentation.
DNA fragmentation
fallopian tube
oviducts
spermatozoa
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
27
12
2021
received:
15
10
2021
accepted:
11
01
2022
pubmed:
12
3
2022
medline:
21
5
2022
entrez:
11
3
2022
Statut:
ppublish
Résumé
The present study investigated the effects of DNA fragmentation of spermatozoa on the growth factors expression by a human oviduct epithelial cell line (OE-E6/E7). Two separate groups were examined in this study. The cell line was cultured in the presence of spermatozoa with normal DNA fragmentation index (DFI) or abnormal DFI. Total RNA from the cell line in each group was isolated, and relative expression of objective genes was analysed using PCR array. Also, the concentration of VEGF, BMP-2, BMP-7 and MSTN in the supernatant of cell culture was analysed by the ELISA method. The PCR array analysis revealed that most of the growth factors had been upregulated in the abnormal group. However, the differences between groups were statistically significant (p < 0.05) for five genes, including VEGF-A, BMP-2, BMP-6, BMP-7 and OSM. Furthermore, MSTN was the only gene that down-regulated significantly under the influence of the spermatozoa with abnormal DFI. Moreover, the results of ELISA analysis were in agreement with the data of the PCR array. It has been concluded that DNA fragmentation in human spermatozoa can probably change regular events throughout the oviducts. Consequently, the genes of interest may change sperm function and probably its fate in the female reproductive tract.
Substances chimiques
Bone Morphogenetic Protein 7
0
Intercellular Signaling Peptides and Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14378Subventions
Organisme : Royan Institute for Reproductive Biomedicine
Informations de copyright
© 2022 Wiley-VCH GmbH.
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