Cu(ii)-based DNA labeling identifies the structural link between transcriptional activation and termination in a metalloregulator.
Journal
Chemical science
ISSN: 2041-6520
Titre abrégé: Chem Sci
Pays: England
ID NLM: 101545951
Informations de publication
Date de publication:
09 Feb 2022
09 Feb 2022
Historique:
received:
24
11
2021
accepted:
16
01
2022
entrez:
14
3
2022
pubmed:
15
3
2022
medline:
15
3
2022
Statut:
epublish
Résumé
Understanding the structural and mechanistic details of protein-DNA interactions that lead to cellular defence against toxic metal ions in pathogenic bacteria can lead to new ways of combating their virulence. Herein, we examine the Copper Efflux Regulator (CueR) protein, a transcription factor which interacts with DNA to generate proteins that ameliorate excess free Cu(i). We exploit site directed Cu(ii) labeling to measure the conformational changes in DNA as a function of protein and Cu(i) concentration. Unexpectedly, the EPR data indicate that the protein can bend the DNA at high protein concentrations
Identifiants
pubmed: 35282619
doi: 10.1039/d1sc06563g
pii: d1sc06563g
pmc: PMC8827015
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1693-1697Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
The authors declare no competing financial interests.
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