Combined proteomic strategies for in-depth venomic analysis of the beaked sea snake (Hydrophis schistosus) from Songkhla Lake, Thailand.
Beaked sea snake
Hydrophis schistosus
Proteomics
Transcriptomics
Venom
Journal
Journal of proteomics
ISSN: 1876-7737
Titre abrégé: J Proteomics
Pays: Netherlands
ID NLM: 101475056
Informations de publication
Date de publication:
15 05 2022
15 05 2022
Historique:
received:
27
09
2021
revised:
22
02
2022
accepted:
07
03
2022
pubmed:
15
3
2022
medline:
17
5
2022
entrez:
14
3
2022
Statut:
ppublish
Résumé
This study focuses on comprehensive characterization of the venom proteome of the beaked sea snake (Hydrophis schistosus) from Songkhla Lake, Thailand. H. schistosus can be considered as the deadliest sea snake commonly found in the Pacific and Indian oceans. Their envenomation causes muscular paralysis and rhabdomyolysis. To develop effective treatment for this snakebite, it is necessary to understand the detailed venom composition. In this study, multiple mass spectrometry-based approaches were employed. Bottom-up proteomics revealed that tryptic digestion in-solution provided a higher number of toxin proteins identified and a larger sequence coverage, compared to in-gel digestion. In addition, a venom gland transcriptome-derived database was constructed and used as a reference, which 43 known and novel toxin proteins were identified using this database and the UniProtKB. Three-finger toxin and phospholipase A
Identifiants
pubmed: 35283353
pii: S1874-3919(22)00082-3
doi: 10.1016/j.jprot.2022.104559
pii:
doi:
Substances chimiques
Elapid Venoms
0
Proteome
0
Toxins, Biological
0
Phospholipases A2
EC 3.1.1.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104559Informations de copyright
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