Long-Term Cultivation of Human Atrial Myocardium.
calcium imaging
confocal microscopy
gene expression
human atrium
in vitro
refractory period
tissue culture
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2022
2022
Historique:
received:
19
12
2021
accepted:
25
01
2022
entrez:
14
3
2022
pubmed:
15
3
2022
medline:
15
3
2022
Statut:
epublish
Résumé
Organotypic culture of human ventricular myocardium is emerging in basic and translational cardiac research. However, few institutions have access to human ventricular tissue, whereas atrial tissue is more commonly available and important for studying atrial physiology. This study presents a method for long-term cultivation of beating human atrial myocardium. After written informed consent, tissues from the right-atrial appendage were obtained from patients with sinus rhythm undergoing open heart surgery with cardiopulmonary bypass. Trabeculae (pectinate muscles) prepared from the samples were installed into cultivation chambers at 37°C with a diastolic preload of 500 μN. After 2 days with 0.5 Hz pacing, stimulation frequency was set to 1 Hz. Contractile force was monitored continuously. Beta-adrenergic response, refractory period (RP) and maximum captured frequency (f
Identifiants
pubmed: 35283779
doi: 10.3389/fphys.2022.839139
pmc: PMC8905341
doi:
Types de publication
Journal Article
Langues
eng
Pagination
839139Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2022 Klumm, Heim, Fiegle, Weyand, Volk and Seidel.
Déclaration de conflit d'intérêts
TS is shareholder of InVitroSys GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nat Protoc. 2021 Oct;16(10):4650-4675
pubmed: 34400840
Front Physiol. 2018 Oct 04;9:1380
pubmed: 30337881
J Appl Physiol (1985). 1997 Aug;83(2):652-60
pubmed: 9262464
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
J Pharmacol Exp Ther. 1982 Jun;221(3):806-14
pubmed: 7086688
Front Cardiovasc Med. 2020 Mar 31;7:43
pubmed: 32296716
Biotechniques. 2021 May;70(5):271-277
pubmed: 34000816
Nat Protoc. 2017 Dec;12(12):2623-2639
pubmed: 29189769
Nat Commun. 2019 Jan 10;10(1):117
pubmed: 30631059
Ann Biomed Eng. 2016 May;44(5):1436-1448
pubmed: 26399990
Heart. 2004 Apr;90(4):400-5
pubmed: 15020515
J Vis Exp. 2017 Jun 4;(124):
pubmed: 28605368
Theranostics. 2021 Apr 15;11(13):6138-6153
pubmed: 33995650
Circulation. 1992 May;85(5):1743-50
pubmed: 1572031
Am J Physiol Heart Circ Physiol. 2007 Jul;293(1):H660-9
pubmed: 17220183
Am J Cardiol. 1992 Feb 15;69(5):513-7
pubmed: 1736617
Neuroscience. 1999 Mar;89(1):91-100
pubmed: 10051219
Thorac Cardiovasc Surg. 1999 Aug;47(4):244-50
pubmed: 10522795
J Gen Physiol. 1995 Jan;105(1):1-19
pubmed: 7730787
Eur Heart J. 1996 Jun;17(6):940-50
pubmed: 8781835
Tissue Eng. 2003 Dec;9(6):1243-53
pubmed: 14670112
Cardiovasc Res. 2020 Dec 1;116(14):2207-2215
pubmed: 32966582
J Vis Exp. 2011 Sep 13;(55):
pubmed: 21946907
Toxicol Appl Pharmacol. 2020 Nov 1;406:115213
pubmed: 32877659
Cell. 2019 Feb 7;176(4):913-927.e18
pubmed: 30686581
J Pharmacol Methods. 1983 Apr;9(2):127-35
pubmed: 6855250
Prog Biophys Mol Biol. 2017 Nov;130(Pt B):302-314
pubmed: 28709857
Cell Physiol Biochem. 2009;24(5-6):527-36
pubmed: 19910693
Circulation. 2004 Sep 14;110(11):1358-63
pubmed: 15313939
J Thorac Cardiovasc Surg. 1994 Mar;107(3):764-75
pubmed: 8127106
Ann Biomed Eng. 1999 Jul-Aug;27(4):563-71
pubmed: 10468240
Eur Heart J. 2020 Jul 1;41(25):2405-2408
pubmed: 31711161
Nat Commun. 2019 May 15;10(1):2168
pubmed: 31092830
Circ Res. 2000 May 26;86(10):1093-9
pubmed: 10827140
Pilot Feasibility Stud. 2017 Dec 20;3:74
pubmed: 29276625
Transplantation. 2000 Apr 15;69(7):1310-22
pubmed: 10798746
Biotechnol Annu Rev. 2005;11:127-52
pubmed: 16216776
Am J Physiol Heart Circ Physiol. 2020 Sep 1;319(3):H519-H530
pubmed: 32734816
Am J Physiol Heart Circ Physiol. 2014 Jul 1;307(1):H73-9
pubmed: 24791783
J Vis Exp. 2020 May 10;(159):
pubmed: 32449745
Am J Physiol Cell Physiol. 2003 Jul;285(1):C171-82
pubmed: 12672651
Future Cardiol. 2009 Sep;5(5):425-30
pubmed: 19715406
Eur J Histochem. 2014 Dec 17;58(4):2448
pubmed: 25578975
Front Physiol. 2020 Mar 13;11:182
pubmed: 32231589
Circulation. 2017 Apr 25;135(17):1632-1645
pubmed: 28073805
Am J Physiol Heart Circ Physiol. 2000 Jan;278(1):H249-55
pubmed: 10644605
J Pharmacol Exp Ther. 1991 Jan;256(1):318-24
pubmed: 1988662
Circ Arrhythm Electrophysiol. 2015 Feb;8(1):32-41
pubmed: 25523945
Circ Res. 2019 Aug 30;125(6):628-642
pubmed: 31310161
Exp Physiol. 2008 Mar;93(3):370-82
pubmed: 18156167
Prog Biophys Mol Biol. 2019 Jul;144:139-150
pubmed: 29960680
Am J Physiol Heart Circ Physiol. 2002 Jul;283(1):H13-21
pubmed: 12063269
Cardiovasc Res. 2012 Jan 1;93(1):50-9
pubmed: 21972180
Cell Physiol Biochem. 2005;16(1-3):127-32
pubmed: 16121041
Circ Res. 1989 Nov;65(5):1441-9
pubmed: 2805252