Identification of extracellular vesicles from J strain and wild isolate of Mycoplasma hyopneumoniae.
Autogenous vaccine
Enzootic pneumonia
Extracellular vesicle
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
19
09
2021
accepted:
23
02
2022
pubmed:
15
3
2022
medline:
3
6
2022
entrez:
14
3
2022
Statut:
ppublish
Résumé
Respiratory diseases constitute a major health challenge for the worldwide pork industry. Porcine enzootic pneumonia (PES) is caused by Mycoplasma hyopneumoniae (Mhyo). Mycoplasmas have the ability to produce extracellular vesicles (EVs), which can be useful for pathogenicity studies and as delivery systems for vaccines. The aim of this study was to demonstrate and compare, under laboratory conditions, EVs produced by Mhyo strain J and wild isolate in stressed and non-stressed in vitro conditions. Using differential centrifugation, density gradient ultracentrifugation, and transmission electron microscopy, the ability of Mhyo strains to produce EVs was demonstrated under favorable and unfavorable conditions.
Identifiants
pubmed: 35286665
doi: 10.1007/s42770-022-00726-0
pii: 10.1007/s42770-022-00726-0
pmc: PMC9151953
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1081-1084Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : APQ-01327-14
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
ID : PPM 00618-17
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 304727/2016-4
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
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