Advancing respiratory-cardiovascular physiology with the working heart-brainstem preparation over 25 years.
Kölliker-Fuse
cardiac ganglion
cardiac vagus
chemoreceptor
eupnoea
gasp
hypertension
hypoxia
pre-Bötzinger complex
respiratory rhythm generation
sympathetic
sympathetic-respiratory coupling
Journal
The Journal of physiology
ISSN: 1469-7793
Titre abrégé: J Physiol
Pays: England
ID NLM: 0266262
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
20
10
2021
accepted:
04
03
2022
pubmed:
17
3
2022
medline:
3
5
2022
entrez:
16
3
2022
Statut:
ppublish
Résumé
Twenty-five years ago, a new physiological preparation called the working heart-brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some of the progress made with the WHBP, some advantages and disadvantages along with potential future applications, and provide photographs and technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of the brain for whole cell recording and an uncompromised use of pharmacological agents akin to in vitro approaches. The preparation has revealed novel mechanistic insights into, for example, the generation of distinct respiratory rhythms, the neurogenesis of sympathetic activity, coupling between respiration and the heart and circulation, hypothalamic and spinal control mechanisms, and peripheral and central chemoreceptor mechanisms. Insights have been gleaned into diseases such as hypertension, heart failure and sleep apnoea. Findings from the in situ preparation have been ratified in conscious in vivo animals and when tested have translated to humans. We conclude by discussing potential future applications of the WHBP including two-photon imaging of peripheral and central nervous systems and adoption of pharmacogenetic tools that will improve our understanding of physiological mechanisms and reveal novel mechanisms that may guide new treatment strategies for cardiorespiratory diseases.
Identifiants
pubmed: 35294064
doi: 10.1113/JP281953
pmc: PMC9322470
doi:
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2049-2075Subventions
Organisme : Intramural Research Program of the NIH, NINDS
Organisme : NIBIB NIH HHS
ID : U01 EB021960
Pays : United States
Organisme : NIH HHS
ID : U01 EB021960
Pays : United States
Organisme : Wellcome Trust Clinical research fellowship
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Canada Institute of Health Research
ID : CIHR- 201603PJT/366421
Informations de copyright
© 2022 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.
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