Laser microscopy acquisition and analysis of premotor synapses in the murine spinal cord.
Cell Biology
Microscopy
Model Organisms
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
18
3
2022
pubmed:
19
3
2022
medline:
23
4
2022
Statut:
epublish
Résumé
Loss of synapses on spinal motor neurons is a major feature of several neurodegenerative diseases; however, analyzing these premotor synapses is challenging because of their small size and high density. This protocol describes confocal and Stimulated Emission Depletion (STED) imaging of murine spinal premotor synapses and their segment-specific quantification by confocal microscopy. We detail the preparation of spinal cord segments, followed by image acquisition and analysis. This protocol enables in-depth analysis of pathological changes in spinal premotor synapses during neurodegeneration. For complete details on the use and execution of this protocol, please refer to Buettner et al. (2021).
Identifiants
pubmed: 35300003
doi: 10.1016/j.xpro.2022.101236
pii: S2666-1667(22)00116-2
pmc: PMC8920933
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101236Subventions
Organisme : NINDS NIH HHS
ID : R01 NS078375
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA027079
Pays : United States
Informations de copyright
© 2022 Leipzig University.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
iScience. 2021 Oct 30;24(11):103376
pubmed: 34825141
Cell Rep. 2020 Jul 28;32(4):107968
pubmed: 32726631
J Neurosci. 2014 Mar 5;34(10):3475-92
pubmed: 24599449
J Neurosci. 2021 Jan 13;41(2):376-389
pubmed: 33219005
Proc Natl Acad Sci U S A. 2021 Mar 2;118(9):
pubmed: 33622791
Elife. 2021 Apr 27;10:
pubmed: 33904397
Nat Neurosci. 2017 Jul;20(7):905-916
pubmed: 28504671
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13588-93
pubmed: 19651609
Cell. 2018 Feb 8;172(4):706-718.e15
pubmed: 29398114
Cell Rep. 2019 Dec 17;29(12):3885-3901.e5
pubmed: 31851921
J Exp Med. 2020 Aug 3;217(8):
pubmed: 32484501
Nat Neurosci. 2016 Oct;19(10):1311-20
pubmed: 27526206
Nat Neurosci. 2018 Jan;21(1):41-49
pubmed: 29230050
Cell Rep. 2017 Dec 26;21(13):3767-3780
pubmed: 29281826
Neural Dev. 2009 Dec 02;4:42
pubmed: 19954518
Neuron. 2011 Feb 10;69(3):453-67
pubmed: 21315257
J Neurosci. 2006 Dec 20;26(51):13297-310
pubmed: 17182780