Effect of serum sample storage temperature on metabolomic and proteomic biomarkers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 03 2022
17 03 2022
Historique:
received:
08
12
2021
accepted:
08
03
2022
entrez:
18
3
2022
pubmed:
19
3
2022
medline:
6
5
2022
Statut:
epublish
Résumé
Prospective biomarker studies can be used to identify biomarkers predictive of disease onset. However, if serum biomarkers are measured years after their collection, the storage conditions might affect analyte concentrations. Few data exists concerning which metabolites and proteins are affected by storage at - 20 °C vs - 80 °C. Our objectives were to document analytes affected by storage of serum samples at - 20 °C vs - 80 °C, and to identify those indicative of the storage temperature. We utilized liquid chromatography tandem mass spectrometry and Luminex to quantify 300 analytes from serum samples of 16 Finnish individuals with type 1 diabetes, with split-aliquot samples stored at - 80 °C and - 20 °C for a median of 4.2 years. Results were validated in 315 Finnish and 916 Scottish individuals with type 1 diabetes, stored at - 20 °C and at - 80 °C, respectively. After quality control, we analysed 193 metabolites and proteins of which 120 were apparently unaffected and 15 clearly susceptible to storage at - 20 °C vs - 80 °C. Further, we identified serum glutamate/glutamine ratio greater than 0.20 as a biomarker of storage at - 20 °C vs - 80 °C. The results provide a catalogue of analytes unaffected and affected by storage at - 20 °C vs - 80 °C and biomarkers indicative of sub-optimal storage.
Identifiants
pubmed: 35301383
doi: 10.1038/s41598-022-08429-0
pii: 10.1038/s41598-022-08429-0
pmc: PMC8930974
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4571Subventions
Organisme : Chief Scientist Office
ID : ETM/47
Pays : United Kingdom
Organisme : Chief Scientist Office
ID : Ref. ETM/47
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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