Quantification of tear glucose levels and their correlation with blood glucose levels in dogs.
blood glucose
diabetes mellitus
dog
hyperglycaemia
tear collection
tear glucose
Journal
Veterinary medicine and science
ISSN: 2053-1095
Titre abrégé: Vet Med Sci
Pays: England
ID NLM: 101678837
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
pubmed:
20
3
2022
medline:
23
7
2022
entrez:
19
3
2022
Statut:
ppublish
Résumé
No previous studies have quantified tear glucose (TG) levels in dogs or compared changes in TG and blood glucose (BG) concentrations. To quantify TG concentration and evaluate its correlation with BG level in dogs. Twenty repetitive tests were performed in alternate eyes of four dogs, with a minimum washout period of 1 week. Tears and blood were collected at 30-min intervals with successive glucose injections (1 g/kg) every 30 min. Cross-correlations of BG and TG levels were assessed. The delay and association between TG and corresponding BG levels were analysed for each dog; samples were collected at 5-min intervals. The tears were collected using microcapillary tubes. Collected tears and blood were analysed for glucose concentration using a colorimetric assay and commercially available glucometer, respectively. The average baseline BG and TG levels were 4.76 ± 0.58 and 0.39 ± 0.04 mmol/L, respectively. Even with highly fluctuating BG levels, a significant cross-correlation coefficient (r = 0.86, p < 0.05) was observed between changes of BG and TG levels. The delay time between BG and TG levels was 10 min. On average, BG levels were 16.34 times higher than TG levels. There was strong correlation between BG and TG levels (r Canine TG concentrations have not been quantified previously. Our findings suggest preliminary data for future research on TG levels in dogs and show TG measurement could be used to screen for diabetes mellitus in dogs.
Sections du résumé
BACKGROUND
No previous studies have quantified tear glucose (TG) levels in dogs or compared changes in TG and blood glucose (BG) concentrations.
OBJECTIVE
To quantify TG concentration and evaluate its correlation with BG level in dogs.
METHODS
Twenty repetitive tests were performed in alternate eyes of four dogs, with a minimum washout period of 1 week. Tears and blood were collected at 30-min intervals with successive glucose injections (1 g/kg) every 30 min. Cross-correlations of BG and TG levels were assessed. The delay and association between TG and corresponding BG levels were analysed for each dog; samples were collected at 5-min intervals. The tears were collected using microcapillary tubes. Collected tears and blood were analysed for glucose concentration using a colorimetric assay and commercially available glucometer, respectively.
RESULTS
The average baseline BG and TG levels were 4.76 ± 0.58 and 0.39 ± 0.04 mmol/L, respectively. Even with highly fluctuating BG levels, a significant cross-correlation coefficient (r = 0.86, p < 0.05) was observed between changes of BG and TG levels. The delay time between BG and TG levels was 10 min. On average, BG levels were 16.34 times higher than TG levels. There was strong correlation between BG and TG levels (r
CONCLUSIONS
Canine TG concentrations have not been quantified previously. Our findings suggest preliminary data for future research on TG levels in dogs and show TG measurement could be used to screen for diabetes mellitus in dogs.
Identifiants
pubmed: 35305285
doi: 10.1002/vms3.788
pmc: PMC9297779
doi:
Substances chimiques
Blood Glucose
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1816-1824Informations de copyright
© 2022 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
Références
Acta Ophthalmol (Copenh). 1988 Aug;66(4):407-12
pubmed: 3195321
Diabetes Technol Ther. 2000 Autumn;2(3):461-72
pubmed: 11467349
J Diabetes Sci Technol. 2011 Jan 01;5(1):166-72
pubmed: 21303640
Vet Ophthalmol. 2019 Jul;22(4):470-476
pubmed: 30288908
Med Res Rev. 2020 Nov;40(6):2566-2604
pubmed: 32735080
Biomacromolecules. 2018 Nov 12;19(11):4504-4511
pubmed: 30350599
Prog Retin Eye Res. 2012 Nov;31(6):527-50
pubmed: 22732126
IEEE Eng Med Biol Mag. 1998 May-Jun;17(3):59-63
pubmed: 9604702
Clin Chem. 2007 Jul;53(7):1370-2
pubmed: 17495022
Curr Diabetes Rev. 2012 Jan;8(1):48-54
pubmed: 22414058
Talanta. 2011 Jan 15;83(3):960-5
pubmed: 21147344
J Am Vet Med Assoc. 2000 Oct 15;217(8):1166-73
pubmed: 11043687
J Fluoresc. 2004 Sep;14(5):617-33
pubmed: 15617269
Anal Chem. 2014 Feb 4;86(3):1902-8
pubmed: 24428813
Biomed Microdevices. 2007 Aug;9(4):603-9
pubmed: 17520370
J Vet Intern Med. 2016 Jul;30(4):927-40
pubmed: 27461721
Intensive Care Med. 2005 Oct;31(10):1442-5
pubmed: 16086177
Diabetes Technol Ther. 2012 May;14(5):398-402
pubmed: 22376082
Curr Eye Res. 2006 Nov;31(11):895-901
pubmed: 17114114
Diabetologia. 1996 May;39(5):609-12
pubmed: 8739922
Sci Adv. 2018 Jan 24;4(1):eaap9841
pubmed: 29387797
Vet J. 2021 Apr;270:105611
pubmed: 33641807
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1977 Mar 29;202(1):1-7
pubmed: 300993
Vet Rec. 2005 Apr 9;156(15):467-71
pubmed: 15828742
Biosens Bioelectron. 2013 Nov 15;49:204-9
pubmed: 23747996
Domest Anim Endocrinol. 2014 Apr;47:55-64
pubmed: 24373250
J Pharm Sci. 1973 Jul;62(7):1112-21
pubmed: 4576801
Vet Clin North Am Small Anim Pract. 1995 May;25(3):661-76
pubmed: 7660540
J Ocul Pharmacol Ther. 2019 Nov;35(9):497-502
pubmed: 31381493
Diabetes Technol Ther. 2001 Spring;3(1):81-90
pubmed: 11469711
Chem Commun (Camb). 2016 Jul 28;52(59):9197-204
pubmed: 27327531
J Small Anim Pract. 2003 Oct;44(10):435-42
pubmed: 14582657
Vet Ophthalmol. 2005 Jul-Aug;8(4):215-24
pubmed: 16008700
Vet Med Sci. 2022 Jul;8(4):1816-1824
pubmed: 35305285
Ocul Surf. 2007 Oct;5(4):280-93
pubmed: 17938838
Invest Ophthalmol. 1966 Jun;5(3):264-76
pubmed: 5947945
Diabetes. 2003 Nov;52(11):2790-4
pubmed: 14578298
J Am Soc Mass Spectrom. 2007 Feb;18(2):332-6
pubmed: 17084090
J Diabetes Sci Technol. 2021 Nov;15(6):1320-1325
pubmed: 33095035