Effects of GLUBRAN-2 on the Burst Pressure of Jejunal Loops Thermofused With Vessel Sealing Devices.

Burst pressure Cyanoacrylate Jejunum Radiofrequency vessel sealing device Suture reinforcement Swine

Journal

The Journal of surgical research
ISSN: 1095-8673
Titre abrégé: J Surg Res
Pays: United States
ID NLM: 0376340

Informations de publication

Date de publication:
07 2022
Historique:
received: 27 09 2021
revised: 14 02 2022
accepted: 16 02 2022
pubmed: 21 3 2022
medline: 27 4 2022
entrez: 20 3 2022
Statut: ppublish

Résumé

Off-label use of radiofrequency vessel sealing devices for intestinal thermofusion has been reported as an alternate approach for closing the small and large intestines. The study aimed to evaluate if reinforcing the thermofusion line with a modified N-butyl-2-CyanoAcrylate and methacryloxysulpholane produced improved burst pressure values in ex vivo swine jejunal loops. A suture-less full-thickness jejunal biopsy was performed with different radiofrequency vessel-sealing devices (Ligasure 5 mm: RFVS-1; Atlast 10 mm: RFVS-2; Cayman Maryland: RFVS-3), and reinforcement with modified cyanoacrylate Glubran-2 (G2) at the thermofusion defect was applied. Burst pressure(BP) values were compared with a control group, wherein a cold blade was utilized to obtain the biopsy, followed by the closing of the jejunum with seven Gambee sutures. Seventy (n = 70) jejunal loop samples were distributed into the experimental groups.The RFVS-1 and -2 groups exhibited BP values similar to those of the suture group. The RFVS-3 group showed significantly lower BP values (P < 0.05) than the suture group. Conversely, in the groups wherein G2 was applied, all BP values were comparable to those of the suture group. BP test in the RFVS-3G2 group showed significantly (P < 0.05) higher values in the group using the same instrument without the glue (RFVS-3). G2 has been shown to improve the BP on the defects created by instruments that are not completely efficient in intestinal thermofusion and sealing. This experimental model showed that the performance of full-thickness biopsies with RFVS devices and reinforcement with G2 provide feasible and promising results.

Sections du résumé

BACKGROUND
Off-label use of radiofrequency vessel sealing devices for intestinal thermofusion has been reported as an alternate approach for closing the small and large intestines. The study aimed to evaluate if reinforcing the thermofusion line with a modified N-butyl-2-CyanoAcrylate and methacryloxysulpholane produced improved burst pressure values in ex vivo swine jejunal loops.
MATERIALS AND METHOD
A suture-less full-thickness jejunal biopsy was performed with different radiofrequency vessel-sealing devices (Ligasure 5 mm: RFVS-1; Atlast 10 mm: RFVS-2; Cayman Maryland: RFVS-3), and reinforcement with modified cyanoacrylate Glubran-2 (G2) at the thermofusion defect was applied. Burst pressure(BP) values were compared with a control group, wherein a cold blade was utilized to obtain the biopsy, followed by the closing of the jejunum with seven Gambee sutures.
RESULTS
Seventy (n = 70) jejunal loop samples were distributed into the experimental groups.The RFVS-1 and -2 groups exhibited BP values similar to those of the suture group. The RFVS-3 group showed significantly lower BP values (P < 0.05) than the suture group. Conversely, in the groups wherein G2 was applied, all BP values were comparable to those of the suture group. BP test in the RFVS-3G2 group showed significantly (P < 0.05) higher values in the group using the same instrument without the glue (RFVS-3).
CONCLUSIONS
G2 has been shown to improve the BP on the defects created by instruments that are not completely efficient in intestinal thermofusion and sealing. This experimental model showed that the performance of full-thickness biopsies with RFVS devices and reinforcement with G2 provide feasible and promising results.

Identifiants

pubmed: 35306259
pii: S0022-4804(22)00099-3
doi: 10.1016/j.jss.2022.02.030
pii:
doi:

Substances chimiques

Cyanoacrylates 0
glubran 2 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

235-243

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Luca Lacitignola (L)

Dipartimento dell'Emergenze e trapianti di organi (D.E.T.O.), Sezione di Cliniche Veterinarie e produzioni animali, Università degli Studi di Bari "Aldo Moro", Bari, Italy. Electronic address: luca.lacitignola@uniba.it.

Annarita Imperante (A)

Dottorato di ricerca in "Trapianti di Tessuti ed Organi e Terapie Cellulari", Dipartimento dell'Emergenza e dei Trapianti di Organi (DETO), Università degli Studi di Bari "Aldo Moro", Bari, Italy.

Salvatore Desantis (S)

Dipartimento dell'Emergenze e trapianti di organi (D.E.T.O.), Sezione di Cliniche Veterinarie e produzioni animali, Università degli Studi di Bari "Aldo Moro", Bari, Italy.

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Classifications MeSH