Sonoporation of Human Renal Proximal Tubular Epithelial Cells In Vitro to Enhance the Liberation of Intracellular miRNA Biomarkers.
Biomarkers
Kidney disease
Liquid biopsy
Microbubbles
Sonoporation
Ultrasound insonation
miRNA
Journal
Ultrasound in medicine & biology
ISSN: 1879-291X
Titre abrégé: Ultrasound Med Biol
Pays: England
ID NLM: 0410553
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
22
09
2021
revised:
11
01
2022
accepted:
29
01
2022
pubmed:
22
3
2022
medline:
28
4
2022
entrez:
21
3
2022
Statut:
ppublish
Résumé
Ultrasound has previously been demonstrated to non-invasively cause tissue disruption. Small animal studies have demonstrated that this effect can be enhanced by contrast microbubbles and has the potential to be clinically beneficial in techniques such as targeted drug delivery or enhancing liquid biopsies when a physical biopsy may be inappropriate. Cavitating microbubbles in close proximity to cells increases membrane permeability, allowing small intracellular molecules to leak into the extracellular space. This study sought to establish whether cavitating microbubbles could liberate cell-specific miRNAs, augmenting biomarker detection for non-invasive liquid biopsies. Insonating human polarized renal proximal tubular epithelial cells (RPTECs), in the presence of SonoVue microbubbles, revealed that cellular health could be maintained while achieving the release of miRNAs, miR-21, miR-30e, miR-192 and miR-194 (respectively, 10.9-fold, 7.17-fold, 5.95-fold and 5.36-fold). To examine the mechanism of release, RPTECs expressing enhanced green fluorescent protein were generated and the protein successfully liberated. Cell polarization, cellular phenotype and cell viability after sonoporation were measured by a number of techniques. Ultrastructural studies using electron microscopy showed gap-junction disruption and pore formation on cellular surfaces. These studies revealed that cell-specific miRNAs can be non-specifically liberated from RPTECs by sonoporation without a significant decrease in cell viability.
Identifiants
pubmed: 35307235
pii: S0301-5629(22)00041-2
doi: 10.1016/j.ultrasmedbio.2022.01.019
pii:
doi:
Substances chimiques
Biomarkers
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1019-1032Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R505638/1
Pays : United Kingdom
Informations de copyright
Crown Copyright © 2022. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest This work was funded by a BBSRC:National Productivity Investment Fund PhD Scholarship co-funded by GSK (BB/R505638/1) awarded to J.H. and L.D. L.D. is supported by a Senior Kidney Research UK Fellowship (SF_001_20181122).