Persistent SARS-CoV-2 antigen presence in multiple organs of a naturally infected cat from Brazil.

Cats Coronavirus Disease transmission Pets SARS-CoV-2

Journal

The journal of venomous animals and toxins including tropical diseases
ISSN: 1678-9199
Titre abrégé: J Venom Anim Toxins Incl Trop Dis
Pays: Brazil
ID NLM: 101201501

Informations de publication

Date de publication:
2022
Historique:
received: 04 07 2021
accepted: 27 08 2021
entrez: 21 3 2022
pubmed: 22 3 2022
medline: 22 3 2022
Statut: epublish

Résumé

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of the disease coronavirus 2019 (COVID-19) in humans. SARS-CoV-2 has been identified in cats with or without clinical signs. We describe the pathological and molecular findings in a six-month-old asymptomatic cat with SARS-CoV-2 infection from Brazil, belonging to a human family with COVID-19 cases. The pool of nasopharynx and oropharynx swabs at day zero tested positive by RT-qPCR for SARS-CoV-2. No amplification resulted from molecular testing performed on days 7 and 14. The cat was hit by a car and died 43 days after the molecular diagnosis. Immunohistochemistry at The present study has highlighted the possibility that viral antigens can be detected by immunohistochemistry in multiple organs six weeks after infection, although the same tissues tested negative by RT-PCR.

Sections du résumé

Background UNASSIGNED
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of the disease coronavirus 2019 (COVID-19) in humans. SARS-CoV-2 has been identified in cats with or without clinical signs.
Case presentation UNASSIGNED
We describe the pathological and molecular findings in a six-month-old asymptomatic cat with SARS-CoV-2 infection from Brazil, belonging to a human family with COVID-19 cases. The pool of nasopharynx and oropharynx swabs at day zero tested positive by RT-qPCR for SARS-CoV-2. No amplification resulted from molecular testing performed on days 7 and 14. The cat was hit by a car and died 43 days after the molecular diagnosis. Immunohistochemistry at
Conclusion UNASSIGNED
The present study has highlighted the possibility that viral antigens can be detected by immunohistochemistry in multiple organs six weeks after infection, although the same tissues tested negative by RT-PCR.

Identifiants

pubmed: 35310131
doi: 10.1590/1678-9199-JVATITD-2021-0074
pmc: PMC8906534
doi:

Types de publication

Case Reports

Langues

eng

Pagination

e20210074

Déclaration de conflit d'intérêts

Competing interests: The authors declare that they have no competing interests.

Références

Mod Pathol. 2020 Nov;33(11):2092-2103
pubmed: 32741970
Nat Microbiol. 2021 Jan;6(1):73-86
pubmed: 33340034
Emerg Infect Dis. 2021 Feb;27(2):660-663
pubmed: 33496650
Nat Commun. 2020 Nov 27;11(1):6059
pubmed: 33247099
Mod Pathol. 2020 Nov;33(11):2104-2114
pubmed: 32561849
Nature. 2020 Sep;585(7824):268-272
pubmed: 32396922
Mod Pathol. 2021 Sep;34(9):1614-1633
pubmed: 34031537
Vet Pathol. 2008 Jul;45(4):551-62
pubmed: 18587105
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):24790-24793
pubmed: 32948692
J Am Vet Med Assoc. 2021 Nov 1;259(9):1032-1039
pubmed: 34647475
N Engl J Med. 2020 Aug 6;383(6):592-594
pubmed: 32402157
Front Vet Sci. 2020 Oct 27;7:591216
pubmed: 33195627
Emerg Microbes Infect. 2020 Dec;9(1):2322-2332
pubmed: 33028154
Res Vet Sci. 2008 Jun;84(3):494-6
pubmed: 17658563
Cell Res. 2020 Aug;30(8):708-710
pubmed: 32632255
Front Vet Sci. 2020 Aug 13;7:514
pubmed: 32903561
Diagn Histopathol (Oxf). 2021 Mar;27(3):123-127
pubmed: 33312229
Nat Commun. 2021 May 4;12(1):2506
pubmed: 33947851
Science. 2020 May 29;368(6494):1016-1020
pubmed: 32269068

Auteurs

Samar Afif Jarrah (SA)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Louise Bach Kmetiuk (LB)

Graduate College of Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.

Otávio Valério de Carvalho (OV)

Graduate College of Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil.

Alessandra Tammy Hayakawa Ito de Sousa (ATHI)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Valeria Regia Franco Souza (VRF)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Luciano Nakazato (L)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Edson Moleta Colodel (EM)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Andrea Pires Dos Santos (AP)

Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.

Christina Pettan-Brewer (C)

Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, Washington, USA.

Rosane Christine Hahn (RC)

Medical Science Faculty, Federal University of Mato Grosso, Cuiabá, MT, Brazil.

Renata Dezengrini Slhessarenko (RD)

Júlio Muller University Hospital, Federal University of Mato Grosso, Cuiabá, MT, Brazil.

Daniel Guimarães Ubiali (DG)

Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropedica, RJ, Brazil.

Asheley Henrique Barbosa Pereira (AHB)

Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropedica, RJ, Brazil.

Helio Autran de Morais (HA)

Department of Clinical Sciences, Oregon State University, Corvallis, OR, United States.

Alexander Welker Biondo (AW)

Department of Veterinary Medicine, Federal University of Paraná, Curitiba, PR, Brazil.

Valéria Dutra (V)

Department of Veterinary Medicine, College of Veterinary Medicine, Federal University of Mato Grosso, Cuiabá, MG, Brazil.

Classifications MeSH