Generation of inflammation-responsive astrocytes from glial progenitors derived from human pluripotent stem cells.
Cell Differentiation
Cell culture
Neuroscience
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 06 2022
17 06 2022
Historique:
entrez:
22
3
2022
pubmed:
23
3
2022
medline:
23
4
2022
Statut:
epublish
Résumé
Astrocytes are an essential component of the central nervous system for neuronal support and response to injury and disease. Here, we present a protocol to generate glial progenitor cells (GPCs) from human-induced pluripotent stem cells (iPSCs) that can be further differentiated into inflammation-responsive astrocytes. This two-step protocol has the advantage of reducing the time of astrocyte differentiation since GPCs can be frozen and stored. For complete details on the use and execution of this protocol, please refer to Santos et al. (2017) and Vadodaria et al. (2021).
Identifiants
pubmed: 35313707
doi: 10.1016/j.xpro.2022.101261
pii: S2666-1667(22)00141-1
pmc: PMC8933838
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101261Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Stem Cell Reports. 2017 Jun 6;8(6):1757-1769
pubmed: 28591655
Mol Psychiatry. 2021 Jul;26(7):3558-3571
pubmed: 32839513
Stem Cell Reports. 2021 Apr 13;16(4):825-835
pubmed: 33667413
Transl Psychiatry. 2021 Nov 30;11(1):608
pubmed: 34848679