Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 03 2022
21 03 2022
Historique:
received:
24
06
2021
accepted:
22
02
2022
entrez:
22
3
2022
pubmed:
23
3
2022
medline:
13
4
2022
Statut:
epublish
Résumé
Peptidoglycan hydrolases contribute to the generation of helical cell shape in Campylobacter and Helicobacter bacteria, while cytoskeletal or periskeletal proteins determine the curved, vibrioid cell shape of Caulobacter and Vibrio. Here, we identify a peptidoglycan hydrolase in the vibrioid-shaped predatory bacterium Bdellovibrio bacteriovorus which invades and replicates within the periplasm of Gram-negative prey bacteria. The protein, Bd1075, generates cell curvature in B. bacteriovorus by exerting LD-carboxypeptidase activity upon the predator cell wall as it grows inside spherical prey. Bd1075 localizes to the outer convex face of B. bacteriovorus; this asymmetric localization requires a nuclear transport factor 2-like (NTF2) domain at the protein C-terminus. We solve the crystal structure of Bd1075, which is monomeric with key differences to other LD-carboxypeptidases. Rod-shaped Δbd1075 mutants invade prey more slowly than curved wild-type predators and stretch invaded prey from within. We therefore propose that the vibrioid shape of B. bacteriovorus contributes to predatory fitness.
Identifiants
pubmed: 35314810
doi: 10.1038/s41467-022-29007-y
pii: 10.1038/s41467-022-29007-y
pmc: PMC8938487
doi:
Substances chimiques
Peptidoglycan
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1509Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 215025/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 209437/Z/17/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L024209/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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