Ultrasensitive determination of 39 parent and emerging halogenated polycyclic aromatic hydrocarbons in human serum.

Halogenated polycyclic aromatic hydrocarbons (HPAHs) have attracted extensive attention because of their high toxicity and bioaccumulation. However, there has been no report on the content of HPAHs in human tissues and the corresponding analytical method. In this study, a method for the simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 23 HPAHs in human serum was developed and validated. Simple and stable removal of interfering substances in complex serum and the detection of ultra-trace HPAHs are the key difficulties. After 0.5 mL serum was treated with formic acid and 10% isopropanol, samples were prepared by solid phase extraction (SPE) and analyzed by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The recoveries of the method were 65-103%, with low detection limits of 0.001-0.019 ng mL