Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle.
Galectin-1
LGMD2B
Localization
Membrane repair
Muscular dystrophy
Protein labeling
Sarcolemma
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
23
3
2022
pubmed:
24
3
2022
medline:
26
3
2022
Statut:
ppublish
Résumé
Galectin-1 is a small (14.5 kDa) multifunctional protein with cell-cell and cell-ECM adhesion due to interactions with the carbohydrate recognition domain (CRD). In two types of muscular dystrophies, this lectin protein has shown therapeutic properties, including positive regulation of skeletal muscle differentiation and regeneration. Both Duchenne and limb-girdle muscular dystrophy 2B (LGMD2B) are subtypes of muscular dystrophies characterized by deficient membrane repair, muscle weakness, and eventual loss of ambulation. This chapter explains confocal techniques such as laser injury, calcium imaging, and galectin-1 localization to examine the effects of galectin-1 on membrane repair in injured LGMD2B models.
Identifiants
pubmed: 35320552
doi: 10.1007/978-1-0716-2055-7_36
doi:
Substances chimiques
Galectin 1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-683Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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