The Basics of Setting up Successful Teaching Labs and Short-Term Projects with C. elegans.
C. elegans
Chemotaxis
Genetics
Inclusive teaching
Neuroscience
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
23
3
2022
pubmed:
24
3
2022
medline:
26
3
2022
Statut:
ppublish
Résumé
Caenorhabditis elegans is an excellent organism for teaching or doing short-term research projects because of the many freely-available comprehensive resources describing its genome and biology, its short-generation time and the ease of working with it in the lab. However, it can be daunting to begin a short-term project or a teaching lab with an organism with which one has little or no experience. Therefore, in this article, we will discuss the minimal equipment, reagents, and protocols needed to begin doing experiments with C. elegans as well as inclusive teaching practices. We will also describe a teaching laboratory for introductory or advanced neuroscience undergraduate courses where students learn to understand the relationship between genetic mutations and behavior through using chemotaxis assays. In addition, instructions for students for the lab module are provided in Subheading "Example of Teaching Lab Module for Students".
Identifiants
pubmed: 35320558
doi: 10.1007/978-1-0716-2181-3_2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25-41Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
American Association for the Advancement of Science (2011) Vision and Change in Undergraduate Biology Education: A Call to Action. https://live-visionandchange.pantheonsite.io/wp-content/uploads/2013/11/aaas-VISchange-web1113.pdf . Accessed 06 Dec 2020
Bargmann CI (2006) Chemosensation in C. elegans WormBook 1–29. https://doi.org/10.1895/wormbook.1.123.1
Hart AC (2006) Behavior. WormBook 1–67. https://doi.org/10.1895/wormbook.1.87.1
Sengupta P, Chou JH, Bargmann CI (1996) Odr-10 encodes a seven transmembrane domain olfactory receptor required for responses to the odorant diacetyl. Cell 84:899–909
doi: 10.1016/S0092-8674(00)81068-5
Sengupta P, Colbert HA, Bargmann CI (1994) The C. elegans gene odr-7 encodes an olfactory-specific member of the nuclear receptor superfamily. Cell 79:971–980
doi: 10.1016/0092-8674(94)90028-0
Stiernagle T (2006) Maintenance of C. elegans. WormBook 1–11. https://doi.org/10.1895/wormbook.1.101.1
Bargmann CI, Hartwieg E, Horvitz HR (1993) Odorant-selective genes and neurons mediate olfaction in C. elegans. Cell 74:515–527
doi: 10.1016/0092-8674(93)80053-H
Johnson KMS (2019) Implementing inclusive practices in an active learning STEM classroom. Adv Physiol Educ 43:207–210
doi: 10.1152/advan.00045.2019
Prahl K (2017) Best practices for the think-pair-share active-learning technique. Am Biol Teach 79:3–8
doi: 10.1525/abt.2017.79.1.3
Kusumoto H, Tashiro K, Shimaoka S et al (2019) Behavioural response alteration in Caenorhabditis elegans to urine after surgical removal of cancer: nematode-NOSE (N-NOSE) for postoperative evaluation. Biomark Cancer 11:1179299X19896551
doi: 10.1177/1179299X19896551