Restoration of DNA integrity and the cell cycle by electric stimulation in planarian tissues damaged by ionizing radiation.
Planaria
DNA repair
Electric stimulation
Galvanotactic
Neoblasts
Stem cells
Tissue regeneration
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
01 05 2022
01 05 2022
Historique:
received:
19
08
2021
accepted:
05
03
2022
pubmed:
25
3
2022
medline:
18
5
2022
entrez:
24
3
2022
Statut:
ppublish
Résumé
Exposure to high levels of ionizing γ radiation leads to irreversible DNA damage and cell death. Here, we establish that exogenous application of electric stimulation enables cellular plasticity and the re-establishment of stem cell activity in tissues damaged by ionizing radiation. We show that subthreshold direct current stimulation (DCS) rapidly restores pluripotent stem cell populations previously eliminated by lethally γ-irradiated tissues of the planarian flatworm Schmidtea mediterranea. Our findings reveal that DCS enhances DNA repair, transcriptional activity, and cell cycle entry in post-mitotic cells. These responses involve rapid increases in cytosolic Ca2+ concentration through the activation of L-type Cav channels and intracellular Ca2+ stores, leading to the activation of immediate early genes and ectopic expression of stem cell markers in post-mitotic cells. Overall, we show the potential of electric current stimulation to reverse the damaging effects of high-dose γ radiation in adult tissues. Furthermore, our results provide mechanistic insights describing how electric stimulation effectively translates into molecular responses capable of regulating fundamental cellular functions without the need for genetic or pharmacological intervention.
Identifiants
pubmed: 35322853
pii: 274829
doi: 10.1242/jcs.259304
pmc: PMC9264365
pii:
doi:
Substances chimiques
DNA
9007-49-2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Institutes of Health National Institute of General Medical Sciences
ID : R01GM132753
Organisme : National Science Foundation
ID : 1744620
Organisme : University of California Cancer Research Coordinating Committee
ID : CRR-18-525108
Organisme : NIGMS NIH HHS
ID : R01 GM132753
Pays : United States
Organisme : NIH HHS
ID : S10 OD010786
Pays : United States
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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