Conjugated detergent micelles as a platform for IgM purification.
IgM purification
[metal:chelator] complexes
conjugated micelles
hydrophobic amino acids
ligand free
nonchromatographic
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
revised:
31
01
2022
received:
07
01
2022
accepted:
20
03
2022
pubmed:
25
3
2022
medline:
18
6
2022
entrez:
24
3
2022
Statut:
ppublish
Résumé
Immunoglobulin M (IgM) antibodies hold promise as anticancer drugs and as agents for promoting immune homeostasis. This promise has not been realized due to low expression levels in mammalian cells producing IgM class antibodies, and the failure of protein A chromatography for IgM purification. Here, we describe a nonchromatographic platform for quantitatively capturing IgMs at neutral pH, which is then recovered with 86%-94% yield and >95% purity at pH 3. The platform contains micelles conjugated with the [(bathophenanthroline)
Identifiants
pubmed: 35324016
doi: 10.1002/bit.28089
pmc: PMC9325453
doi:
Substances chimiques
Antibodies, Monoclonal
0
Detergents
0
Immunoglobulin M
0
Micelles
0
Staphylococcal Protein A
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1997-2003Informations de copyright
© 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
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