MRGPRX2-Mediated Degranulation of Human Skin Mast Cells Requires the Operation of G
ERK1/2
FcεRI
G proteins
MAP kinases
MRGPRX2
PI3K/AKT
degranulation
mast cells
signal transduction
skin
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
10 03 2022
10 03 2022
Historique:
received:
15
01
2022
revised:
01
03
2022
accepted:
08
03
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
14
4
2022
Statut:
epublish
Résumé
The recent discovery of MRGPRX2 explains mast cell (MC)-dependent symptoms independently of FcεRI-activation. Because of its novelty, signaling cascades triggered by MRGPRX2 are rudimentarily understood, especially in cutaneous MCs, by which MRGPRX2 is chiefly expressed. Here, MCs purified from human skin were used following preculture or ex vivo and stimulated by FcεRI-aggregation or MRGPRX2 agonists (compound 48/80, Substance P) in the presence/absence of inhibitors. Degranulation was assessed by β-hexosaminidase or histamine release. Phosphorylation events were studied by immunoblotting. As a G protein-coupled receptor, MRGPRX2 signals by activating G proteins; however, their nature has remained controversial. In skin MCs, G
Identifiants
pubmed: 35326404
pii: cells11060953
doi: 10.3390/cells11060953
pmc: PMC8946553
pii:
doi:
Substances chimiques
MRGPRX2 protein, human
0
Nerve Tissue Proteins
0
Receptors, G-Protein-Coupled
0
Receptors, IgE
0
Receptors, Neuropeptide
0
GTP-Binding Proteins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : BA-3769/4-1
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