Osmotic Stress Interferes with DNA Damage Response and H2AX Phosphorylation in Human Keratinocytes.
DNA repair disorders
UV radiation
gene regulation
keratinocytes
osmotic stress
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 03 2022
11 03 2022
Historique:
received:
08
02
2022
revised:
02
03
2022
accepted:
04
03
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
14
4
2022
Statut:
epublish
Résumé
The human skin and in particular its outermost layer, the epidermis, protects the body from potentially harmful substances, radiation as well as excessive water loss. However, the interference between the various stress responses of the epidermal keratinocytes, which often occur simultaneously, is largely unknown. The focus of this study was to investigate the interference between osmotic stress and DNA damage response. In addition to revealing the already well-described regulation of diverse gene sets, for example, cellular processes such as transcription, translation, and metabolic pathways (e.g., the KEGG citrate cycle and Reactome G2/M checkpoints), gene expression analysis of osmotically stressed keratinocytes revealed an influence on the transcription of genes also related to UV-induced DNA damage response. A gene network regulating the H2AX phosphorylation was identified to be regulated by osmotic stress. To analyze and test the interference between osmotic stress and DNA damage response, which can be triggered by UV stress on the one hand and oxidative stress on the other, in more detail, primary human keratinocytes were cultured under osmotic stress conditions and subsequently exposed to UV light and H
Identifiants
pubmed: 35326410
pii: cells11060959
doi: 10.3390/cells11060959
pmc: PMC8946833
pii:
doi:
Substances chimiques
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Bavarian State Ministry of Education and Culture, Science and Art (BMUKK)
ID : VII.2.F1116.CO/28/3
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