A Temporary Pause in the Replication Licensing Restriction Leads to Rereplication during Early Human Cell Differentiation.
CDT1
GMNN
fiber combing
gene amplification
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
21 03 2022
21 03 2022
Historique:
received:
18
02
2022
revised:
18
03
2022
accepted:
20
03
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
12
4
2022
Statut:
epublish
Résumé
Gene amplifications in amphibians and flies are known to occur during development and have been well characterized, unlike in mammalian cells, where they are predominantly investigated as an attribute of tumors. Recently, we first described gene amplifications in human and mouse neural stem cells, myoblasts, and mesenchymal stem cells during differentiation. The mechanism leading to gene amplifications in amphibians and flies depends on endocycles and multiple origin-firings. So far, there is no knowledge about a comparable mechanism in normal human cells. Here, we describe rereplication during the early myotube differentiation of human skeletal myoblast cells, using fiber combing and pulse-treatment with EdU (5'-Ethynyl-2'-deoxyuridine)/CldU (5-Chlor-2'-deoxyuridine) and IdU (5-Iodo-2'-deoxyuridine)/CldU. We found rereplication during a restricted time window between 2 h and 8 h after differentiation induction. Rereplication was detected in cells simultaneously with the amplification of the
Identifiants
pubmed: 35326512
pii: cells11061060
doi: 10.3390/cells11061060
pmc: PMC8946923
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
Deoxyuridine
W78I7AY22C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
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