Citrus bergamia
antimicrobial activity
fungicidal activity
multi-drug-resistant bacteria
time-kill assay
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
08 Mar 2022
08 Mar 2022
Historique:
received:
01
02
2022
revised:
24
02
2022
accepted:
04
03
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
26
3
2022
Statut:
epublish
Résumé
The inappropriate use of antibiotics has increased selective pressure and the spread of multi-drug-resistant (MDR) pathogens, which reduces the possibility of effective treatment. A potential alternative therapeutic approach may be represented by essential oils, such as the distilled extract of bergamot ( This work aimed to evaluate the kinetics of the bactericidal and fungicidal activity of the distilled extract of bergamot on MDR bacteria and fungi from clinical specimens using the Our results demonstrated that the distilled extract of bergamot exhibited significant antimicrobial activity and a specific bactericidal effect against the bacterial and fungal strains tested. Furthermore, confocal microscope images clearly showed compromised membrane integrity, damage and cell death in bacterial samples treated with the distilled extract of bergamot. In addition, progressive alterations in cell-wall composition, cytoplasmic material and nucleus structure triggered by exposure to the distilled extract of bergamot were identified in the fungal samples considered. Our data suggest that the use of essential oils, such as distilled extract of bergamot (
Sections du résumé
BACKGROUND
BACKGROUND
The inappropriate use of antibiotics has increased selective pressure and the spread of multi-drug-resistant (MDR) pathogens, which reduces the possibility of effective treatment. A potential alternative therapeutic approach may be represented by essential oils, such as the distilled extract of bergamot (
METHODS
METHODS
This work aimed to evaluate the kinetics of the bactericidal and fungicidal activity of the distilled extract of bergamot on MDR bacteria and fungi from clinical specimens using the
RESULTS
RESULTS
Our results demonstrated that the distilled extract of bergamot exhibited significant antimicrobial activity and a specific bactericidal effect against the bacterial and fungal strains tested. Furthermore, confocal microscope images clearly showed compromised membrane integrity, damage and cell death in bacterial samples treated with the distilled extract of bergamot. In addition, progressive alterations in cell-wall composition, cytoplasmic material and nucleus structure triggered by exposure to the distilled extract of bergamot were identified in the fungal samples considered.
CONCLUSIONS
CONCLUSIONS
Our data suggest that the use of essential oils, such as distilled extract of bergamot (
Identifiants
pubmed: 35326824
pii: antibiotics11030361
doi: 10.3390/antibiotics11030361
pmc: PMC8944555
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministry of Education, Universities and Research
ID : PON-MIUR 03PE000_78_1, PONMIUR 03PE000_78_2; POR Calabria FESR FSE 2014-2020 Asse 12-Azioni 10.5.6 e 10.5.12. POR Calabria FESR FSE 2014-2020 azione 1.5.1: progetto AgrInfra Calabria
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