One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors.
Cell biology
Molecular biology
Stem cells research
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
15 Apr 2022
15 Apr 2022
Historique:
received:
13
07
2021
revised:
10
01
2022
accepted:
24
02
2022
entrez:
25
3
2022
pubmed:
26
3
2022
medline:
26
3
2022
Statut:
epublish
Résumé
Retinal dystrophies (RDs) lead to irreversible vision impairment with no radical treatment. Although photoreceptor cells (PRCs) differentiated from human induced pluripotent stem cells (iPSCs) are essential for the study of RDs as a scalable source, current differentiation methods for PRCs require multiple steps. To address these issues, we developed a method to generate PRCs from human iPSCs by introducing the transcription factors, CRX and NEUROD1. This approach enabled us to generate induced photoreceptor-like cells (iPRCs) expressing PRC markers. Single-cell RNA sequencing revealed the transcriptome of iPRCs in which the genes associated with phototransduction were expressed. Generated iPRCs exhibited their functional properties in calcium imaging. Furthermore, light-induced damage on iPRCs was inhibited by an antioxidant compound. This simple approach would facilitate the availability of materials for PRC-related research and provide a useful application for disease modeling and drug discovery.
Identifiants
pubmed: 35330684
doi: 10.1016/j.isci.2022.103987
pii: S2589-0042(22)00257-7
pmc: PMC8938283
doi:
Types de publication
Journal Article
Langues
eng
Pagination
103987Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Prog Retin Eye Res. 1998 Apr;17(2):175-205
pubmed: 9695792
J Neurosci. 2008 Jan 16;28(3):749-56
pubmed: 18199774
Stem Cells. 2017 May;35(5):1176-1188
pubmed: 28220575
Stem Cells. 2015 Aug;33(8):2416-30
pubmed: 25827910
Am J Pathol. 2009 Jun;174(6):2310-23
pubmed: 19435787
Nat Commun. 2015 Feb 19;6:6286
pubmed: 25695148
Front Genet. 2019 Apr 05;10:308
pubmed: 31024622
Nature. 2008 Oct 2;455(7213):627-32
pubmed: 18754011
Epigenetics Chromatin. 2018 Aug 1;11(1):42
pubmed: 30068366
Cell Mol Life Sci. 2013 Jul;70(14):2519-32
pubmed: 23064704
Cornea. 2019 Nov;38 Suppl 1:S34-S41
pubmed: 31403532
Nat Genet. 1999 Dec;23(4):466-70
pubmed: 10581037
Cell Stem Cell. 2012 Jun 14;10(6):771-785
pubmed: 22704518
Cell. 1997 Nov 14;91(4):531-41
pubmed: 9390562
Sci Rep. 2014 Jan 08;4:3594
pubmed: 24399248
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
Nat Commun. 2017 Aug 16;8(1):271
pubmed: 28814713
PLoS One. 2018 Jul 6;13(7):e0200210
pubmed: 29979748
Pflugers Arch. 2014 Jul;466(7):1241-57
pubmed: 24142069
Exp Eye Res. 2008 Feb;86(2):344-54
pubmed: 18163989
Stem Cell Reports. 2018 Sep 11;11(3):665-680
pubmed: 30100409
Cell Biol Int. 2018 May;42(5):608-614
pubmed: 29377330
Sci Rep. 2017 Apr 10;7(1):766
pubmed: 28396597
Int J Mol Sci. 2019 May 23;20(10):
pubmed: 31126147
Nature. 2009 Dec 3;462(7273):587-94
pubmed: 19956253
Cell. 2020 Sep 17;182(6):1623-1640.e34
pubmed: 32946783
Oncotarget. 2016 Jul 5;7(27):42314-42329
pubmed: 27275539
Ther Adv Ophthalmol. 2018 Apr 26;10:2515841418774433
pubmed: 29998222
Arch Ophthalmol. 2002 Nov;120(11):1435-42
pubmed: 12427055
JAMA Ophthalmol. 2016 Aug 1;134(8):924-7
pubmed: 27281386
Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1679-84
pubmed: 17242361
Neuron. 1997 Nov;19(5):1017-30
pubmed: 9390516
Cell Stem Cell. 2016 Jun 2;18(6):769-781
pubmed: 27151457
PLoS One. 2012;7(4):e35611
pubmed: 22558175
Stem Cell Reports. 2018 Apr 10;10(4):1267-1281
pubmed: 29526738
Protein Cell. 2014 Jan;5(1):48-58
pubmed: 24474194
Genes Cells. 2014 Mar;19(3):198-208
pubmed: 24456169
Heliyon. 2018 Mar 01;4(2):e00544
pubmed: 29560458
Development. 1999 Jan;126(1):23-36
pubmed: 9834183
Handb Exp Pharmacol. 2017;242:337-367
pubmed: 28035529
Methods Mol Biol. 2016;1357:111-31
pubmed: 26025620
Biol Open. 2016 Jun 15;5(6):709-19
pubmed: 27170256
Hum Mol Genet. 2017 Jul 1;26(13):2480-2492
pubmed: 28444310
Nat Rev Neurosci. 2010 Aug;11(8):563-76
pubmed: 20648062
Sci Rep. 2014 Jun 09;4:5223
pubmed: 24909301
J Cereb Blood Flow Metab. 2020 Sep;40(9):1739-1751
pubmed: 32423328
Stem Cell Rev Rep. 2018 Aug;14(4):463-483
pubmed: 29675776
Stem Cells. 2018 May;36(5):709-722
pubmed: 29327488
EBioMedicine. 2019 Jan;39:562-574
pubmed: 30502055
Clin Neurophysiol. 2020 Jun;131(6):1383-1398
pubmed: 31866339
Nature. 2020 May;581(7806):83-88
pubmed: 32376950
Adv Drug Deliv Rev. 2019 Feb 1;140:33-50
pubmed: 29777757