A comparison of two different analytical workflows to determine the venom proteome composition of Naja kaouthia from North-East India and immunological profiling of venom against commercial antivenoms.
Indian monocled cobra
LC-MS/MS analysis
Venom proteomics
Venom-antivenom interaction
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 May 2022
31 May 2022
Historique:
received:
20
01
2022
revised:
15
03
2022
accepted:
15
03
2022
pubmed:
26
3
2022
medline:
11
5
2022
entrez:
25
3
2022
Statut:
ppublish
Résumé
The Indian monocled cobra (Naja kaouthia) is one of the most prevalent venomous snakes in northeast India (NEI) and is the cause of many fatalities. The composition of NEI N. kaouthia venom (NkV) was deciphered using two different proteomic approaches: (i) 1D SDS-PAGE coupled to label-free quantification of protein bands using stringent identification criteria and (ii) reversed-phase high-performance liquid chromatography (RP-HPLC) followed by quantification based on area under the RP-HPLC peaks. The proteomic data from both strategies were compared. Proteomic analyses from both workflows identified 32 proteins (toxins) distributed over 10-14 snake venom protein families in NEI NkV. The relative abundances of the venom proteins determined from the analytical workflows coincided with the densitometry band intensities of the NEI NkV. Phospholipase A
Identifiants
pubmed: 35331793
pii: S0141-8130(22)00565-7
doi: 10.1016/j.ijbiomac.2022.03.095
pii:
doi:
Substances chimiques
Antivenins
0
Elapid Venoms
0
Proteome
0
Toxins, Biological
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
275-287Informations de copyright
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