Extracellular Vesicles Derived from Mesenchymal Stromal Cells Delivered during Hypothermic Oxygenated Machine Perfusion Repair Ischemic/Reperfusion Damage of Kidneys from Extended Criteria Donors.
COXIV-1
HGF
VEGF
caspase 3
expanded criteria donors
extracellular vesicles
ischemia/reperfusion injury
kidney transplantation
machine perfusion
mesenchymal stromal cells
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
22 Feb 2022
22 Feb 2022
Historique:
received:
20
01
2022
revised:
16
02
2022
accepted:
18
02
2022
entrez:
26
3
2022
pubmed:
27
3
2022
medline:
27
3
2022
Statut:
epublish
Résumé
The poor availability of kidney for transplantation has led to a search for new strategies to increase the donor pool. The main option is the use of organs from extended criteria donors. We evaluated the effects of hypothermic oxygenated perfusion (HOPE) with and without extracellular vesicles (EV) derived from mesenchymal stromal cells on ischemic/reperfusion injury of marginal kidneys unsuitable for transplantation. For normothermic reperfusion (NR), we used artificial blood as a substitute for red blood cells. We evaluated the global renal ischemic dam-age score (GRS), analyzed the renal ultrastructure (RU), cytochrome c oxidase (COX) IV-1 (a mitochondrial distress marker), and caspase-3 renal expression, the tubular cell proliferation index, hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) tissue levels, and effluent lactate and glucose levels. HOPE+EV kidneys had lower GRS and better RU, higher COX IV-1 expression and HGF and VEGF levels and lower caspase-3 expression than HOPE kidneys. During NR, HOPE+EV renal effluent had lower lactate release and higher glucose levels than HOPE renal effluent, suggesting that the gluconeogenesis system in HOPE+EV group was pre-served. In conclusion, EV delivery during HOPE can be considered a new organ preservation strategy for increasing the donor pool and improving transplant outcome.
Identifiants
pubmed: 35336724
pii: biology11030350
doi: 10.3390/biology11030350
pmc: PMC8945029
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Health System Research
ID : RF-2016-02364732
Références
Am J Transplant. 2020 Jan;20 Suppl s1:20-130
pubmed: 31898417
Biomaterials. 2019 Mar;197:146-160
pubmed: 30654160
Am J Transplant. 2015 Jan;15(1):161-9
pubmed: 25521639
Int J Mol Sci. 2021 Jul 02;22(13):
pubmed: 34281230
Stem Cells. 2020 Apr;38(4):469-476
pubmed: 31828924
Clin Transplant. 2021 Nov;35(11):e14448
pubmed: 34363412
Int J Artif Organs. 2004 Dec;27(12):1019-29
pubmed: 15645611
Nat Rev Drug Discov. 2013 May;12(5):347-57
pubmed: 23584393
Tissue Eng Part A. 2017 Nov;23(21-22):1262-1273
pubmed: 28471327
Ann Transplant. 2010 Jul-Sep;15(3):35-43
pubmed: 20877265
Stem Cell Rev Rep. 2017 Apr;13(2):226-243
pubmed: 28070858
J Am Soc Nephrol. 2001 Mar;12(3):589-597
pubmed: 11181808
Toxics. 2016;4(1):
pubmed: 27471729
J Am Soc Nephrol. 2006 Jun;17(6):1503-20
pubmed: 16707563
PLoS One. 2019 Oct 23;14(10):e0224066
pubmed: 31644544
J Heart Lung Transplant. 2015 Jan;34(1):113-121
pubmed: 25447577
Biotechnol Prog. 2004 Jul-Aug;20(4):1225-32
pubmed: 15296452
Liver Transpl. 2018 Apr;24(4):528-538
pubmed: 29281862
Transplantation. 2017 Nov;101(11):2746-2756
pubmed: 28520579
Clin Sci (Lond). 2011 Jan;120(1):25-36
pubmed: 20583996
Mayo Clin Proc. 2013 Oct;88(10):1174-9
pubmed: 24079687
Crit Care Clin. 2009 Apr;25(2):373-82, Table of Contents
pubmed: 19341914
Bioconjug Chem. 1997 Jan-Feb;8(1):23-30
pubmed: 9026031
Transplantation. 2020 Mar;104(3):482-489
pubmed: 31568396
Curr Drug Discov Technol. 2012 Sep;9(3):158-65
pubmed: 21745179
Lancet. 2004 Nov 13-19;364(9447):1814-27
pubmed: 15541456
Free Radic Biol Med. 2018 Aug 20;124:299-310
pubmed: 29920341
Methods Enzymol. 2008;437:311-28
pubmed: 18433635
Int Urol Nephrol. 2013 Dec;45(6):1809-13
pubmed: 23054314
Artif Organs. 2020 Jan;44(1):81-90
pubmed: 31368159
Br J Surg. 2013 Jul;100(8):991-1001
pubmed: 23754643
Stem Cell Res Ther. 2015 Sep 03;6:153
pubmed: 26334526
Biomacromolecules. 2005 Jul-Aug;6(4):2172-81
pubmed: 16004460
J Clin Invest. 1984 Oct;74(4):1156-64
pubmed: 6434591
Cell. 2007 Apr 6;129(1):111-22
pubmed: 17418790
Cells. 2021 Jul 02;10(7):
pubmed: 34359843
Am J Transplant. 2002 Sep;2(8):701-11
pubmed: 12243491
PLoS One. 2016 Feb 11;11(2):e0148542
pubmed: 26866372
Cytokine Growth Factor Rev. 2009 Feb;20(1):77-85
pubmed: 19201250
Anesthesiology. 2009 Nov;111(5):946-63
pubmed: 19858869
J Cell Mol Med. 2017 Dec;21(12):3381-3393
pubmed: 28639291
Transpl Int. 2016 Apr;29(4):403-15
pubmed: 26756928
G Ital Nefrol. 2010 Jan-Feb;27(1):56-68
pubmed: 20191461
Int J Mol Sci. 2019 May 14;20(10):
pubmed: 31091699
World J Transplant. 2015 Jun 24;5(2):52-67
pubmed: 26131407
Bio Protoc. 2019 Dec 20;9(24):
pubmed: 31867411
Clin Transl Med. 2016 Mar;5(1):7
pubmed: 26943717
Am J Transplant. 2016 Sep;16(9):2545-55
pubmed: 26946212
Blood Transfus. 2010 Jun;8 Suppl 3:s59-68
pubmed: 20606751
Transplant Proc. 2010 May;42(4):1331-5
pubmed: 20534294
BMC Infect Dis. 2020 Feb 10;20(1):115
pubmed: 32041547
IUBMB Life. 2011 May;63(5):355-62
pubmed: 21618402
Am J Transplant. 2021 Apr;21(4):1382-1390
pubmed: 32897651
Arch Biochem Biophys. 2011 Jan 1;505(1):42-7
pubmed: 20920461
Biochim Biophys Acta. 2014 Aug;1846(1):75-87
pubmed: 24747178
Am J Transplant. 2019 Apr;19(4):1202-1211
pubmed: 30588774
Am J Case Rep. 2017 Sep 20;18:1009-1013
pubmed: 28928357
Cryobiology. 2010 Jul;60(3 Suppl):S20-35
pubmed: 19857479
Artif Organs. 2018 Jul;42(7):714-722
pubmed: 29602202
Ann Transplant. 2012 Jan-Mar;17(1):35-42
pubmed: 22466907
Nat Cell Biol. 2007 Jun;9(6):654-9
pubmed: 17486113
Adv Exp Med Biol. 2011;701:85-90
pubmed: 21445773
Am J Transplant. 2013 May;13(5):1246-52
pubmed: 23433047
Trends Mol Med. 2010 Oct;16(10):447-57
pubmed: 20708968
J Transl Med. 2015 Oct 16;13:329
pubmed: 26474973
Transpl Int. 2018 Nov;31(11):1233-1244
pubmed: 29957863
Biochim Biophys Acta. 2008 Oct;1784(10):1402-9
pubmed: 18405674
Biomater Artif Cells Immobilization Biotechnol. 1992;20(2-4):159-79
pubmed: 1391433
Artif Cells Blood Substit Immobil Biotechnol. 2004;32(3):353-74
pubmed: 15508274
J Thorac Cardiovasc Surg. 2002 Jul;124(1):35-42
pubmed: 12091806
Transplant Proc. 2010 May;42(4):1336-40
pubmed: 20534295
Oxid Med Cell Longev. 2021 Jun 9;2021:5543452
pubmed: 34211627
Surg Gynecol Obstet. 1987 Oct;165(4):343-8
pubmed: 3310285
N Engl J Med. 2006 Jan 26;354(4):343-52
pubmed: 16436766
Cells. 2020 Feb 05;9(2):
pubmed: 32033489
Ann Transplant. 2018 Jan 12;23:34-44
pubmed: 29326416
N Engl J Med. 2011 Feb 17;364(7):656-65
pubmed: 21323543
Growth Factors. 2007 Dec;25(6):382-91
pubmed: 18365869
Cells. 2019 Oct 11;8(10):
pubmed: 31614642
Sci Rep. 2020 Apr 8;10(1):6063
pubmed: 32269237
Nat Commun. 2016 Jun 15;7:11958
pubmed: 27302045
Anesthesiology. 2001 Jun;94(6):1133-8
pubmed: 11465607
Liver Transpl. 2021 Aug;27(8):1130-1143
pubmed: 33835695
Anesth Analg. 2002 Apr;94(4):799-808, table of contents
pubmed: 11916776
Am J Transplant. 2019 Oct;19(10):2814-2824
pubmed: 30938927
Am J Transplant. 2003;3 Suppl 4:114-25
pubmed: 12694055
J Am Soc Nephrol. 2009 May;20(5):1053-67
pubmed: 19389847
Transfusion. 2003 Apr;43(4):509-16
pubmed: 12662285
Stem Cell Res Ther. 2017 Feb 7;8(1):24
pubmed: 28173878
Blood. 2007 Oct 1;110(7):2440-8
pubmed: 17536014
Front Cell Dev Biol. 2020 Mar 24;8:188
pubmed: 32266268
Transfusion. 2004 Oct;44(10):1516-30
pubmed: 15383027
JAMA. 2008 May 21;299(19):2304-12
pubmed: 18443023
J Bioenerg Biomembr. 2016 Feb;48(1):77-85
pubmed: 26782060
Transplant Direct. 2017 Apr 18;3(5):e155
pubmed: 28573190
Bioconjug Chem. 2018 May 16;29(5):1560-1575
pubmed: 29570272
Transplantation. 2006 Dec 15;82(11):1425-8
pubmed: 17164712
Am J Transplant. 2016 Nov;16(11):3282-3285
pubmed: 27273794
Biotechnol Prog. 2011 Jul;27(4):1172-84
pubmed: 21584950
BMC Immunol. 2014 Oct 03;15:44
pubmed: 25277788
Methods Enzymol. 2000;322:183-98
pubmed: 10914015
Anal Chem. 2006 Jul 1;78(13):4634-41
pubmed: 16808476
Am J Nephrol. 2013;37(2):158-66
pubmed: 23392180