Specific overexpression of SIRT1 in mesenchymal stem cells rescues hematopoiesis niche in BMI1 knockout mice through promoting CXCL12 expression.


Journal

International journal of biological sciences
ISSN: 1449-2288
Titre abrégé: Int J Biol Sci
Pays: Australia
ID NLM: 101235568

Informations de publication

Date de publication:
2022
Historique:
received: 15 06 2021
accepted: 08 02 2022
entrez: 28 3 2022
pubmed: 29 3 2022
medline: 16 4 2022
Statut: epublish

Résumé

Osteoblastic lineage cells (OBCs) are bone-building cells and essential component of hematopoietic niche, but mechanisms whereby bone-building and hematopoiesis-supportive activities of OBCs could be regulated simultaneously remain largely unknown. Here we found that B cell-specific Moloney murine leukemia virus integration site 1 (Bmi1) was involved in such a co-regulatory mechanism. In this study, we first found that, accompanied with marked decline of osteogenic activity, the hematopoietic niche in Bmi1 knockout (KO) mice was severely impaired and manifested as CXCL12 expression falls and LSK homing failure; however, intratibial injection with CXCL12 effectively facilitated LSK accumulation in bone marrow of Bmi1 KO mice. To try to rescue these defects in Bmi1 KO mice, we generated Bmi1

Identifiants

pubmed: 35342358
doi: 10.7150/ijbs.63876
pii: ijbsv18p2091
pmc: PMC8935221
doi:

Substances chimiques

Bmi1 protein, mouse 0
Chemokine CXCL12 0
Cxcl12 protein, mouse 0
Proto-Oncogene Proteins 0
Polycomb Repressive Complex 1 EC 2.3.2.27
Sirt1 protein, mouse EC 3.5.1.-
Sirtuin 1 EC 3.5.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2091-2103

Informations de copyright

© The author(s).

Déclaration de conflit d'intérêts

Competing Interests: The authors have declared that no competing interest exists.

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Auteurs

Jinbo Li (J)

Department of Pharmacology, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang, Hebei 050011, China.
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Xing Li (X)

Department of Immuno-oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.

Wen Sun (W)

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Jiao Zhang (J)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Quanquan Yan (Q)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Jun Wu (J)

Department of Human Anatomy, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Jianliang Jin (J)

Department of Human Anatomy, Nanjing Medical University, Nanjing, Jiangsu 210029, China.

Ruinan Lu (R)

Department of Hematology, Jiangsu People's Hospital, Nanjing, Jiangsu 210029, China.

Dengshun Miao (D)

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Department of Plastic Surgery, The Affiliated Friendship Plastic Surgery Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.

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Classifications MeSH