Spectroscopic evidence of fluorescence by 1,8-diazafluoren-9-one aggregates-A prospective new ultrasensitive method for fingerprint trace detection.
1,8-diazafluoren-9-one
DFO
absorption
aggregates in the excited state
fingerprints
fluorescence
friction ridge analysis
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
revised:
09
03
2022
received:
21
01
2022
accepted:
15
03
2022
pubmed:
29
3
2022
medline:
6
7
2022
entrez:
28
3
2022
Statut:
ppublish
Résumé
Friction ridge analysis would not have been one of the most recognized branches of forensics without molecular spectroscopy. The phenomenon of fluorescence is used on daily basis to develop latent fingerprints and to enhance those that are visible. The idea behind the research was to discover selected spectroscopic properties of 1,8-diazafluoren-9-one (DFO) in various environments. This fluorescent compound has been routinely used for decades to develop latent fingerprints due to its numerous advantages, but to this day, it has not been well-understood. Analysis of absorption, fluorescence, and excitation spectra of DFO in ethanol at high dye concentration allowed identification of aggregates in the excited state. A significant influence of the dye concentration on the fluorescence spectra and on the fluorescence excitation spectra was found. In particular, dye-host aggregation was found to be much stronger in a polar solvent. DFO aggregates are strongly fluorescent in ethanol, as can be seen from the steady-state emission spectra. The impact of excitation wavelength on the effect of fingerprint detection is presented. A new reaction medium has been proposed, ethanol, which is nontoxic in relation to the currently used one, methanol. The existence in this medium of DFO aggregates in the excited state, which significantly influences the identification of amino acids present in fingerprint traces, was observed, emitting in a wide spectral range (green light).
Identifiants
pubmed: 35344207
doi: 10.1111/1556-4029.15039
doi:
Substances chimiques
Solvents
0
Ethanol
3K9958V90M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1468-1475Subventions
Organisme : Narodowe Centrum Nauki
ID : 2021/41/B/HS5/03250
Informations de copyright
© 2022 American Academy of Forensic Sciences.
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