Photocontrollable Probes for Mitochondrial Protein Profiling.
mitochondrial proteome
photo-crosslinker
photocontrol
quinone methide
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
18 05 2022
18 05 2022
Historique:
revised:
21
03
2022
received:
31
01
2022
pubmed:
29
3
2022
medline:
21
5
2022
entrez:
28
3
2022
Statut:
ppublish
Résumé
The mitochondrion is the core site of cell signaling, energy metabolism and biosynthesis. Here, taking advantage of activity-based probes, we synthesized two photocontrollable probes (YGH-1 and YGH-2), composed of a mitochondrial localization moiety "triphenylphosphonium", a photo-triggered group to achieve spatially and temporally controlled protein capture, and an alkyne group to enrich the labeled protein. Proteomic validation was further carried out to facilitate identification of the mitochondrial proteome in HeLa cells. The results show that half of the identified protein hits (∼300) labeled by YGH-1 and YGH-2 belong to mitochondria, and are mostly localized in the mitochondrial matrix and inner mitochondrial membrane. Our results provide a new tool for spatial and temporal analysis of subcellular proteomes.
Identifiants
pubmed: 35344259
doi: 10.1002/cbic.202200066
doi:
Substances chimiques
Mitochondrial Proteins
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200066Informations de copyright
© 2022 Wiley-VCH GmbH.
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