The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
07
01
2022
accepted:
11
03
2022
revised:
07
04
2022
pubmed:
29
3
2022
medline:
12
4
2022
entrez:
28
3
2022
Statut:
epublish
Résumé
Despite extensive studies on the curve-shaped bacterium Vibrio cholerae, the causative agent of the diarrheal disease cholera, its virulence-associated regulatory two-component signal transduction system VarS/VarA is not well understood. This pathway, which mainly signals through the downstream protein CsrA, is highly conserved among gamma-proteobacteria, indicating there is likely a broader function of this system beyond virulence regulation. In this study, we investigated the VarA-CsrA signaling pathway and discovered a previously unrecognized link to the shape of the bacterium. We observed that varA-deficient V. cholerae cells showed an abnormal spherical morphology during late-stage growth. Through peptidoglycan (PG) composition analyses, we discovered that these mutant bacteria contained an increased content of disaccharide dipeptides and reduced peptide crosslinks, consistent with the atypical cellular shape. The spherical shape correlated with the CsrA-dependent overproduction of aspartate ammonia lyase (AspA) in varA mutant cells, which likely depleted the cellular aspartate pool; therefore, the synthesis of the PG precursor amino acid meso-diaminopimelic acid was impaired. Importantly, this phenotype, and the overall cell rounding, could be prevented by means of cell wall recycling. Collectively, our data provide new insights into how V. cholerae use the VarA-CsrA signaling system to adjust its morphology upon unidentified external cues in its environment.
Identifiants
pubmed: 35344548
doi: 10.1371/journal.pgen.1010143
pii: PGENETICS-D-22-00020
pmc: PMC8989286
doi:
Substances chimiques
Bacterial Proteins
0
Peptidoglycan
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010143Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 101824/Z/13/Z
Pays : United Kingdom
Organisme : Howard Hughes Medical Institute
ID : 55008726
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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