Epidemiology Mycobacterium tuberculosis Seasonality Transmission Whole genome sequencing

Journal

The Lancet regional health. Europe
ISSN: 2666-7762
Titre abrégé: Lancet Reg Health Eur
Pays: England
ID NLM: 101777707

Informations de publication

Date de publication:
Jun 2022
Historique:
entrez: 29 3 2022
pubmed: 30 3 2022
medline: 30 3 2022
Statut: epublish

Résumé

Over 10-years of whole-genome sequencing (WGS) of Between 1st January 2009 and 15th June 2019, we obtained the first WGS isolate from every patient resident in a postcode district covered by Birmingham's centralised tuberculosis service. Data on patients' sex, country of birth, social risk-factors, anatomical locus of disease, and strain lineage were collected. Poisson harmonic regression was used to assess seasonal variation in case load and a mixed-effects multivariable Cox proportionate hazards model was used to assess risk factors for a future case arising in clusters defined by a 5 single nucleotide polymorphism (SNP) threshold, and by 12 SNPs in a sensitivity analysis. 511/1653 (31%) patients were genomically clustered with another. A seasonal variation in diagnoses was observed, peaking in spring, but only among clustered cases. Risk-factors for a future clustered case included UK-birth (aHR=2·03 (95%CI 1·35-3·04), There is seasonal variation in the diagnosis of genomically clustered, but not non-clustered, cases. Risk factors for clustering include UK-birth, infectious forms of tuberculosis, and infection with lineage 3 or 4. Wellcome Trust, MRC, UKHSA.

Sections du résumé

Background UNASSIGNED
Over 10-years of whole-genome sequencing (WGS) of
Methods UNASSIGNED
Between 1st January 2009 and 15th June 2019, we obtained the first WGS isolate from every patient resident in a postcode district covered by Birmingham's centralised tuberculosis service. Data on patients' sex, country of birth, social risk-factors, anatomical locus of disease, and strain lineage were collected. Poisson harmonic regression was used to assess seasonal variation in case load and a mixed-effects multivariable Cox proportionate hazards model was used to assess risk factors for a future case arising in clusters defined by a 5 single nucleotide polymorphism (SNP) threshold, and by 12 SNPs in a sensitivity analysis.
Findings UNASSIGNED
511/1653 (31%) patients were genomically clustered with another. A seasonal variation in diagnoses was observed, peaking in spring, but only among clustered cases. Risk-factors for a future clustered case included UK-birth (aHR=2·03 (95%CI 1·35-3·04),
Interpretation UNASSIGNED
There is seasonal variation in the diagnosis of genomically clustered, but not non-clustered, cases. Risk factors for clustering include UK-birth, infectious forms of tuberculosis, and infection with lineage 3 or 4.
Funding UNASSIGNED
Wellcome Trust, MRC, UKHSA.

Identifiants

pubmed: 35345560
doi: 10.1016/j.lanepe.2022.100361
pii: S2666-7762(22)00054-0
pmc: PMC8956939
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100361

Subventions

Organisme : Medical Research Council
ID : MR/J011398/1
Pays : United Kingdom

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

No authors declare any conflict of interests.

Références

Eur Respir J. 2019 Nov 28;54(5):
pubmed: 31467121
Clin Infect Dis. 2012 Jun;54(11):1553-60
pubmed: 22474225
Lancet Infect Dis. 2013 Feb;13(2):137-46
pubmed: 23158499
J Infect. 2019 Dec;79(6):572-581
pubmed: 31585190
PLoS One. 2012;7(7):e41253
pubmed: 22911768
EBioMedicine. 2018 Aug;34:122-130
pubmed: 30077721
Lancet. 2004 Oct 30-Nov 5;364(9445):1613-4
pubmed: 15519633
J Infect Dis. 2014 Sep 1;210(5):774-83
pubmed: 24596279
J Clin Microbiol. 2018 Jul 26;56(8):
pubmed: 29875188
Am J Respir Crit Care Med. 2017 Jun 1;195(11):1519-1527
pubmed: 27997216
J Clin Microbiol. 2015 Apr;53(4):1137-43
pubmed: 25631807
N Engl J Med. 2020 Jul 23;383(4):359-368
pubmed: 32706534
BMJ. 2018 Aug 23;362:k2738
pubmed: 30139910
PLoS One. 2013;8(3):e57752
pubmed: 23483924
J Glob Infect Dis. 2011 Jan;3(1):46-55
pubmed: 21572609
PLoS Med. 2019 Oct 31;16(10):e1002961
pubmed: 31671150
Thorax. 2018 Aug;73(8):769-775
pubmed: 29674389
Nat Genet. 2018 Jun;50(6):849-856
pubmed: 29785015
Genome Res. 2011 Jun;21(6):936-9
pubmed: 20980556
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943

Auteurs

Timothy M Walker (TM)

Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, UK.
Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam.

Marc Choisy (M)

Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, UK.
Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam.

Martin Dedicoat (M)

University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.

Philip G Drennan (PG)

Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, UK.
Oxford University Hospitals NHS Foundation Trust, UK.

David Wyllie (D)

TB Unit and National Mycobacterial Reference Service, UK Health Security Agency, UK.

Fan Yang-Turner (F)

NIHR Oxford Biomedical Research Centre, University of Oxford, UK.

Derrick W Crook (DW)

Oxford University Hospitals NHS Foundation Trust, UK.
NIHR Oxford Biomedical Research Centre, University of Oxford, UK.

Esther R Robinson (ER)

TB Unit and National Mycobacterial Reference Service, UK Health Security Agency, UK.

A Sarah Walker (AS)

NIHR Oxford Biomedical Research Centre, University of Oxford, UK.

E Grace Smith (EG)

TB Unit and National Mycobacterial Reference Service, UK Health Security Agency, UK.

Timothy E A Peto (TEA)

Oxford University Hospitals NHS Foundation Trust, UK.
NIHR Oxford Biomedical Research Centre, University of Oxford, UK.

Classifications MeSH