Antioxidant defense system in the prefrontal cortex of chronically stressed rats treated with lithium.
Antioxidant enzymes
Chronic restraint stress
Lithium
Prefrontal cortex
Rats
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2022
2022
Historique:
received:
06
10
2021
accepted:
07
02
2022
entrez:
29
3
2022
pubmed:
30
3
2022
medline:
30
3
2022
Statut:
epublish
Résumé
This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS). The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals. These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
Sections du résumé
Background
This study aimed to investigate the effects of lithium treatment on gene expression and activity of the prefrontal antioxidant enzymes: copper, zinc superoxide dismutase (SOD1), manganes superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPx) in animals exposed to chronic restraint stress (CRS).
Methods
The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assays of enzyme activities.
Results
We found that lithium treatment decreased gene expression of SOD2, as well as the activities of SOD1 and SOD2 in chronically stressed rats to the levels found in unstressed animals. However, lithium treatment in animals exposed to CRS increased prefrontal GPx activity to the levels found in unstressed animals.
Conclusions
These findings confirm that treatment with lithium induced the modulation of prefrontal antioxidant status in chronically stressed rats. Our results may be very important in biomedical research for understanding the role of lithium in maintaining the stability of prefrontal antioxidant defense system in neuropsychiatric disorders caused by chronic stress.
Identifiants
pubmed: 35345589
doi: 10.7717/peerj.13020
pii: 13020
pmc: PMC8957266
doi:
Substances chimiques
Antioxidants
0
Lithium
9FN79X2M3F
Superoxide Dismutase-1
EC 1.15.1.1
Superoxide Dismutase
EC 1.15.1.1
Lithium Compounds
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e13020Informations de copyright
© 2022 Gavrilović et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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