Structures and immunomodulatory activity of one galactose- and arabinose-rich polysaccharide from Sambucus adnata.

Animals Arabinose Galactose Mice Polysaccharides / chemistry RAW 264.7 Cells Sambucus
Immunomodulatory activity Polysaccharide Sambucus adnata Structure

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
15 May 2022
Historique:
received: 12 01 2022
revised: 02 03 2022
accepted: 21 03 2022
pubmed: 30 3 2022
medline: 27 4 2022
entrez: 29 3 2022
Statut: ppublish

Résumé

One galactose- and arabinose-rich polysaccharide isolated from Sambucus adnata was named SPS-1, which had an average molecular weight 138.52 kDa, and was composed of L-rhamnose, D-glucuronic acid, D-galacturonic acid, D-galactose, and L-arabinose in a molar ratio of 0.6:0.4:0.1:4.9:4.0. The primary structure of SPS-1 was further analyzed through methylation and NMR spectroscopy. The results showed that SPS-1 had the structural characteristics of AG-II pectin. The immunoactivity test showed that SPS-1 activated the phosphorylation of MAPKs-related proteins and further elevated the expression levels of related nuclear transcription factors (IκBα and NF-κB p65) in the cells through the TLR2 and MyD88/TRAF6-dependent pathway, thereby significantly enhancing the phagocytosis of macrophages and stimulating the secretion of NO, IL-1β, IL-6, and TNF-α, which activated the RAW264.7 cells. Therefore, SPS-1, acting as an immunomodulator, is a potential drug for immunological diseases.

Identifiants

DOI: 10.1016/j.ijbiomac.2022.03.132 PMID: 35346678
pubmed: 35346678
pii: S0141-8130(22)00602-X
doi: 10.1016/j.ijbiomac.2022.03.132
pii:
doi:

Substances chimiques

Polysaccharides 0
Arabinose B40ROO395Z
Galactose X2RN3Q8DNE

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

730-740

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Lei Yuan (L)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; Biotechnology Center, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China. Electronic address: yuanl@xza.edu.cn.

Zheng-Chang Zhong (ZC)

The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Yu Liu (Y)

The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Hong Quan (H)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; Research Institute of Plateau Ecology, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Ya-Zhou Lu (YZ)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Er-Hao Zhang (EH)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Hao Cai (H)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Lian-Qiang Li (LQ)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China.

Xiao-Zhong Lan (XZ)

The Provincial and Ministerial Co-Founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China; The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China. Electronic address: lanxiaozhong@xza.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Structures and immunomodulatory activity of...
questionsmedicales.fr › Glucides › Sucres › Oses › Pentoses › Arabinose › Structures and immunomodulatory activity of...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Galactose › Structures and immunomodulatory activity of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Structures and immunomodulatory activity of...
questionsmedicales.fr › Glucides › Polyosides › Structures and immunomodulatory activity of...
questionsmedicales.fr › Cellules › Cellules RAW 264.7 › Structures and immunomodulatory activity of...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Dipsacales › Adoxaceae › Sambucus › Structures and immunomodulatory activity of...