Imaging Regional Lung Structure and Function in Small Animals Using Synchrotron Radiation Phase-Contrast and K-Edge Subtraction Computed Tomography.

computed tomography pulmonary function regional blood flow respiration artificial synchrotrons

Journal

Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006

Informations de publication

Date de publication:
2022
Historique:
received: 30 11 2021
accepted: 14 02 2022
entrez: 30 3 2022
pubmed: 31 3 2022
medline: 31 3 2022
Statut: epublish

Résumé

Synchrotron radiation offers unique properties of coherence, utilized in phase-contrast imaging, and high flux as well as a wide energy spectrum which allow the selection of very narrow energy bands of radiation, used in K-edge subtraction imaging (KES) imaging. These properties extend X-ray computed tomography (CT) capabilities to quantitatively assess lung morphology, and to map regional lung ventilation, perfusion, inflammation, aerosol particle distribution and biomechanical properties, with microscopic spatial resolution. Four-dimensional imaging, allows the investigation of the dynamics of regional lung functional parameters simultaneously with structural deformation of the lung as a function of time. These techniques have proven to be very useful for revealing the regional differences in both lung structure and function which is crucial for better understanding of disease mechanisms as well as for evaluating treatment in small animal models of lung diseases. Here, synchrotron radiation imaging methods are described and examples of their application to the study of disease mechanisms in preclinical animal models are presented.

Identifiants

pubmed: 35350681
doi: 10.3389/fphys.2022.825433
pmc: PMC8957951
doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

825433

Informations de copyright

Copyright © 2022 Bayat, Fardin, Cercos-Pita, Perchiazzi and Bravin.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Sam Bayat (S)

Univ. Grenoble Alpes, Inserm UA07 STROBE Laboratory, University of Grenoble Alpes, Grenoble, France.
Department of Pulmonology and Clinical Physiology, Grenoble University Hospital, Grenoble, France.

Luca Fardin (L)

European Synchrotron Radiation Facility, Grenoble, France.

José Luis Cercos-Pita (JL)

Hedenstierna Laboratory, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.

Gaetano Perchiazzi (G)

Hedenstierna Laboratory, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.

Alberto Bravin (A)

Department of Physics, University of Milano-Bicocca, Milan, Italy.

Classifications MeSH