Rapid detection and discrimination of potentially toxigenic Corynebacterium ulcerans and Corynebacterium pseudotuberculosis by multiplex real-time PCR and amplicon melting curve analysis.

Corynebacterium / genetics Corynebacterium Infections / diagnosis Corynebacterium pseudotuberculosis / genetics Diphtheria / microbiology Diphtheria Toxin / genetics Humans Real-Time Polymerase Chain Reaction
Corynebacterium diphtheriae Corynebacterium pseudotuberculosis Corynebacterium ulcerans Diphtheria toxin Multiplex real-time PCR

Journal

Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883

Informations de publication

Date de publication:
04 2022
Historique:
received: 15 11 2021
revised: 16 03 2022
accepted: 23 03 2022
pubmed: 31 3 2022
medline: 14 4 2022
entrez: 30 3 2022
Statut: ppublish

Résumé

We developed a multiplex real-time PCR assay with amplicon melting curve analysis to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay should be a valuable tool for identification of potentially toxigenic C. ulcerans.

Identifiants

DOI: 10.1016/j.mimet.2022.106454 PMID: 35351489
pubmed: 35351489
pii: S0167-7012(22)00049-5
doi: 10.1016/j.mimet.2022.106454
pii:
doi:

Substances chimiques

Diphtheria Toxin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106454

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Jun Kawase (J)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan. Electronic address: kawase-jun@pref.shimane.lg.jp.

Tomotake Sakai (T)

Shimane Prefectural Hamada Public Health Center, 254 kataniwa, Hamada City, Shimane 697-0041, Japan.

Masaaki Iwaki (M)

Department of Bacteriology II, Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama City, Tokyo 208-0011, Japan.

Kaoru Umeda (K)

Division of Microbiology, Osaka Institute of Public Health, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.

Aiko Fukuma (A)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

Naoki Fujisawa (N)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

Yuta Kawakami (Y)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

Hiroki Hayashi (H)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

Mieko Wada (M)

Division of Bacteriology, Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, City, Shimane 690-0122, Japan.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Corynebacterium › Rapid detection and discrimination of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à Actinomycetales › Infections à Corynebacterium › Rapid detection and discrimination of...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Corynebacterium › Corynebacterium pseudotuberculosis › Rapid detection and discrimination of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à Actinomycetales › Infections à Corynebacterium › Diphtérie › Rapid detection and discrimination of...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Toxine diphtérique › Rapid detection and discrimination of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Rapid detection and discrimination of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Rapid detection and discrimination of...