BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.

Animals Chromatin / genetics Dosage Compensation, Genetic Epigenesis, Genetic Female Mice RNA, Long Noncoding / genetics X Chromosome / genetics X Chromosome Inactivation / genetics

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
29 03 2022
Historique:
received: 10 01 2021
accepted: 10 03 2022
entrez: 30 3 2022
pubmed: 31 3 2022
medline: 14 4 2022
Statut: epublish

Résumé

The process of epigenetic silencing, while fundamentally important, is not yet completely understood. Here we report a replenishable female mouse embryonic stem cell (mESC) system, Xmas, that allows rapid assessment of X chromosome inactivation (XCI), the epigenetic silencing mechanism of one of the two X chromosomes that enables dosage compensation in female mammals. Through a targeted genetic screen in differentiating Xmas mESCs, we reveal that the BAF complex is required to create nucleosome-depleted regions at promoters on the inactive X chromosome during the earliest stages of establishment of XCI. Without this action gene silencing fails. Xmas mESCs provide a tractable model for screen-based approaches that enable the discovery of unknown facets of the female-specific process of XCI and epigenetic silencing more broadly.

Identifiants

DOI: 10.1038/s41467-022-29333-1 PMID: 35351876 PMC: PMC8964718
pubmed: 35351876
doi: 10.1038/s41467-022-29333-1
pii: 10.1038/s41467-022-29333-1
pmc: PMC8964718
doi:

Substances chimiques

Chromatin 0
RNA, Long Noncoding 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1658

Informations de copyright

© 2022. The Author(s).

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Auteurs

Andrew Keniry (A)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. keniry@wehi.edu.au.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia. keniry@wehi.edu.au.
ORCID: 0000-0002-4061-9540

Natasha Jansz (N)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-3319-8493

Linden J Gearing (LJ)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0003-3508-3056

Iromi Wanigasuriya (I)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Joseph Chen (J)

Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, VIC, Australia.
Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.

Christian M Nefzger (CM)

Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
The University of Queensland, Institute for Molecular Bioscience, St Lucia, QLD, Australia.
ORCID: 0000-0003-4631-5633

Peter F Hickey (PF)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-8153-6258

Quentin Gouil (Q)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-5142-7886

Joy Liu (J)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Kelsey A Breslin (KA)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
ORCID: 0000-0003-4616-8215

Megan Iminitoff (M)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Tamara Beck (T)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Andres Tapia Del Fierro (A)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-5800-0885

Lachlan Whitehead (L)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-4388-9642

Andrew Jarratt (A)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Sarah A Kinkel (SA)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Phillippa C Taberlay (PC)

School of Medicine, College of Health and Medicine, University of Tasmania, Hobart, TAS, Australia.
ORCID: 0000-0002-5298-8730

Tracy Willson (T)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Miha Pakusch (M)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Matthew E Ritchie (ME)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
ORCID: 0000-0002-7383-0609

Douglas J Hilton (DJ)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.

Jose M Polo (JM)

Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia.
Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, VIC, Australia.
Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
ORCID: 0000-0002-2531-778X

Marnie E Blewitt (ME)

The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. blewitt@wehi.edu.au.
The Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia. blewitt@wehi.edu.au.
ORCID: 0000-0002-2984-1474

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Compensation de dosage génétique › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › ARN long non codant › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Chromosomes › Chromosomes sexuels › Chromosome X › BAF complex-mediated chromatin relaxation is...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Compensation de dosage génétique › Inactivation du chromosome X › BAF complex-mediated chromatin relaxation is...