Dual-contrast micro-CT enables cartilage lesion detection and tissue condition evaluation ex vivo.
articular cartilage
bismuth nanoparticles
contrast-enhanced computed tomography
horse
ioxaglate
osteoarthritis
Journal
Equine veterinary journal
ISSN: 2042-3306
Titre abrégé: Equine Vet J
Pays: United States
ID NLM: 0173320
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
20
05
2021
accepted:
10
03
2022
pubmed:
31
3
2022
medline:
9
2
2023
entrez:
30
3
2022
Statut:
ppublish
Résumé
Post-traumatic osteoarthritis is a frequent joint disease in the horse. Currently, equine medicine lacks effective methods to diagnose the severity of chondral defects after an injury. To investigate the capability of dual-contrast-enhanced computed tomography (dual-CECT) for detection of chondral lesions and evaluation of the severity of articular cartilage degeneration in the equine carpus ex vivo. Pre-clinical experimental study. In nine Shetland ponies, blunt and sharp grooves were randomly created (in vivo) in the cartilage of radiocarpal and middle carpal joints. The contralateral joint served as control. The ponies were subjected to an 8-week exercise protocol and euthanised 39 weeks after surgery. CECT scanning (ex vivo) of the joints was performed using a micro-CT scanner 1 hour after an intra-articular injection of a dual-contrast agent. The dual-contrast agent consisted of ioxaglate (negatively charged, q = -1) and bismuth nanoparticles (BiNPs, q = 0, diameter ≈ 0.2 µm). CECT results were compared to histological cartilage proteoglycan content maps acquired using digital densitometry. BiNPs enabled prolonged visual detection of both groove types as they are too large to diffuse into the cartilage. Furthermore, proportional ioxaglate diffusion inside the tissue allowed differentiation between the lesion and ungrooved articular cartilage (3 mm from the lesion and contralateral joint). The mean ioxaglate partition in the lesion was 19 percentage points higher (P < 0.001) when compared with the contralateral joint. The digital densitometry and the dual-contrast CECT findings showed good subjective visual agreement. Ex vivo study protocol and a low number of investigated joints. The dual-CECT methodology, used in this study for the first time to image whole equine joints, is capable of effective lesion detection and simultaneous evaluation of the condition of the articular cartilage.
Sections du résumé
BACKGROUND
BACKGROUND
Post-traumatic osteoarthritis is a frequent joint disease in the horse. Currently, equine medicine lacks effective methods to diagnose the severity of chondral defects after an injury.
OBJECTIVES
OBJECTIVE
To investigate the capability of dual-contrast-enhanced computed tomography (dual-CECT) for detection of chondral lesions and evaluation of the severity of articular cartilage degeneration in the equine carpus ex vivo.
STUDY DESIGN
METHODS
Pre-clinical experimental study.
METHODS
METHODS
In nine Shetland ponies, blunt and sharp grooves were randomly created (in vivo) in the cartilage of radiocarpal and middle carpal joints. The contralateral joint served as control. The ponies were subjected to an 8-week exercise protocol and euthanised 39 weeks after surgery. CECT scanning (ex vivo) of the joints was performed using a micro-CT scanner 1 hour after an intra-articular injection of a dual-contrast agent. The dual-contrast agent consisted of ioxaglate (negatively charged, q = -1) and bismuth nanoparticles (BiNPs, q = 0, diameter ≈ 0.2 µm). CECT results were compared to histological cartilage proteoglycan content maps acquired using digital densitometry.
RESULTS
RESULTS
BiNPs enabled prolonged visual detection of both groove types as they are too large to diffuse into the cartilage. Furthermore, proportional ioxaglate diffusion inside the tissue allowed differentiation between the lesion and ungrooved articular cartilage (3 mm from the lesion and contralateral joint). The mean ioxaglate partition in the lesion was 19 percentage points higher (P < 0.001) when compared with the contralateral joint. The digital densitometry and the dual-contrast CECT findings showed good subjective visual agreement.
MAIN LIMITATIONS
CONCLUSIONS
Ex vivo study protocol and a low number of investigated joints.
CONCLUSIONS
CONCLUSIONS
The dual-CECT methodology, used in this study for the first time to image whole equine joints, is capable of effective lesion detection and simultaneous evaluation of the condition of the articular cartilage.
Identifiants
pubmed: 35353399
doi: 10.1111/evj.13573
pmc: PMC10084070
doi:
Substances chimiques
Ioxaglic Acid
Z40X7EI2AF
Contrast Media
0
Types de publication
Case Reports
Langues
eng
Sous-ensembles de citation
IM
Pagination
315-324Subventions
Organisme : Dutch Arthritis Association grant
ID : LLP-22
Organisme : Academy of Finland
ID : 307932
Organisme : Academy of Finland
ID : 314412
Organisme : Academy of Finland
ID : 324529
Organisme : the University of Eastern Finland's Doctoral Programme in Science, Technology and Computing (SCITECO)
Organisme : Emil Aaltosen Säätiö
ID : 200016
Organisme : the Research Committee of the Kuopio University Hospital Catchment Area for the State Research Funding
ID : 5041769
Organisme : NWO Graduate Programme Grant
ID : 022.005.018
Organisme : Vilho, Yrjö and Kalle Väisälä Foundation of the Finnish Academy of Science and Letters
Organisme : Alfred Kordelinin Säätiö
ID : 190111
Informations de copyright
© 2022 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
Références
Cartilage. 2017 Oct;8(4):391-399
pubmed: 28934883
PLoS One. 2015 Feb 13;10(2):e0115089
pubmed: 25680102
Equine Vet J. 2018 Sep;50(5):564-579
pubmed: 29344988
Osteoarthritis Cartilage. 2014 Jun;22(6):869-78
pubmed: 24769230
Osteoarthritis Cartilage. 2021 Mar;29(3):423-432
pubmed: 33359249
Cartilage. 2012 Oct;3(4):334-41
pubmed: 26069643
J Biomech. 1998 Jun;31(6):511-7
pubmed: 9755035
J Orthop Res. 2014 Mar;32(3):403-12
pubmed: 24249683
Ann Biomed Eng. 2017 Mar;45(3):811-818
pubmed: 27646147
Magn Reson Imaging. 2012 Nov;30(9):1323-41
pubmed: 22770690
J Orthop Res. 2021 Aug;39(8):1647-1657
pubmed: 33104251
J Orthop Res. 2017 Mar;35(3):496-505
pubmed: 27183198
Acta Radiol. 2009 Jan;50(1):78-85
pubmed: 19052932
Osteoarthritis Cartilage. 2004 Mar;12(3):177-90
pubmed: 14972335
Radiology. 2013 Jan;266(1):141-50
pubmed: 23192774
J Bone Joint Surg Am. 1985 Dec;67(9):1336-9
pubmed: 4077904
Osteoarthritis Cartilage. 2019 Aug;27(8):1219-1228
pubmed: 31075424
Radiology. 1987 Mar;162(3):729-33
pubmed: 3544035
Ann Biomed Eng. 2017 Dec;45(12):2857-2866
pubmed: 28924827
Vet Rec. 2005 Oct 15;157(16):470-7
pubmed: 16227382
Osteoarthritis Cartilage. 2011 Aug;19(8):970-6
pubmed: 21549206
J Orthop Res. 2021 Nov;39(11):2363-2375
pubmed: 33368588
J Orthop Res. 2021 Apr;39(4):771-779
pubmed: 32767676
PLoS One. 2018 Apr 25;13(4):e0196203
pubmed: 29694389
Ann Biomed Eng. 2020 Feb;48(2):556-567
pubmed: 31576504
Vet Radiol Ultrasound. 2011 Jan-Feb;52(1):61-70
pubmed: 21322388
Equine Vet J. 2017 Jul;49(4):410-424
pubmed: 28407291
J Orthop Res. 2011 Jun;29(6):802-9
pubmed: 21520254
Clin Orthop Relat Res. 1988 Oct;(235):289-95
pubmed: 3416536
Vet Rec. 2009 Sep 5;165(10):281-8
pubmed: 19734560
ACS Appl Mater Interfaces. 2020 Oct 21;12(42):47233-47244
pubmed: 32970405
J Mech Behav Biomed Mater. 2017 Mar;67:51-60
pubmed: 27987426
Stem Cells Cloning. 2015 Aug 28;8:117-24
pubmed: 26357483
Vet J. 2016 Aug;214:61-71
pubmed: 27387728
Skeletal Radiol. 2011 Sep;40(9):1197-222
pubmed: 21847750
Osteoarthritis Cartilage. 2002 Apr;10(4):282-9
pubmed: 11950251
Equine Vet J. 2023 Mar;55(2):315-324
pubmed: 35353399
N Z Vet J. 2005 Apr;53(2):113-22
pubmed: 15846395
Osteoarthritis Cartilage. 2008 Aug;16(8):919-28
pubmed: 18203630