Protective effect of edaravone on cisplatin-induced injury in rat ovary.
Apoptosis
Cisplatin
Edaravone
Ovarium
Rat
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
31
10
2021
accepted:
16
03
2022
pubmed:
1
4
2022
medline:
1
10
2022
entrez:
31
3
2022
Statut:
ppublish
Résumé
This study was aimed to evaluate the protective effect of edaravone on cisplatin-induced ovarian injury. A total 40 female Wistar-Albino rats were utilized to form four groups: Group 1 (control group) (n = 10), no procedure was performed. Group 2 (cisplatin group) (n = 10), single-dose 7.5 mg/kg cisplatin was administered and no procedure was performed. Group 3 (edaravone group) (n = 10), single-dose 1 mg/kg edaravone was administered and no procedure was performed. Group 4 (cisplatin + edaravone group) (n = 10), single-dose 7.5 mg/kg cisplatin and 1 mg/kg edaravone were administered. Seventy-two hours later, ovaries were surgically extirpated in all groups. Malondialdehyde (MDA) levels and nitric oxide (NO) levels were studied in blood samples. In ovarian tissue samples, DNA damage and apoptosis were assessed using TUNEL method. Ovarian tissue damage was evaluated by immunohistochemical staining with caspase 3 and caspase 8. According to the findings obtained from the study, edaravone showed protective properties on ovarian damage due to cisplatin. MDA and NO levels were significantly higher in cisplatin group than other groups. Histopathological ovarian tissue damage in the cisplatin group was significantly higher than other groups. Similarly, DNA damage and apoptosis were higher in cisplatin group and this difference was found to be statistically significant. The immunohistochemical staining which was done using caspase 3 and caspase 8 was revealed that immunoreactive cells were statistically higher in cisplatin group than cisplatin + edaravone group. Edaravone seems to be effective in prevention of ovarian damage and short-term treatment.
Identifiants
pubmed: 35357583
doi: 10.1007/s00404-022-06538-9
pii: 10.1007/s00404-022-06538-9
doi:
Substances chimiques
Nitric Oxide
31C4KY9ESH
Malondialdehyde
4Y8F71G49Q
Caspase 3
EC 3.4.22.-
Caspase 8
EC 3.4.22.-
Cisplatin
Q20Q21Q62J
Edaravone
S798V6YJRP
Antipyrine
T3CHA1B51H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1673-1678Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Gerald KMcE, Pharm D (2006) AHFS drug information, Bethesda, Maryland
Kayaalp O (2005) Türkiye İlaç Kılavuzu TİK-4 2005 Formülleri. Turgut Yayıncılık, Ankara
Chtourou Y, Aouey B, Kebieche M, Fetoui H (2015) Protective role of naringin against cisplatin induced oxidative stress, inflammatory response and apoptosis in rat striatum via suppressing ROS-mediated NF-κB and P53 signaling pathways. Chem Biol Interact 239:76–86
doi: 10.1016/j.cbi.2015.06.036
Kim JH, Jeong SJ, Kwon HY, Park SY, Lee HJ (2010) Decursin prevents cisplatin-induced apoptosis via the enhancement of antioxidant enzymes in human renal epithelial cells. Biol Pharm Bull 33(8):1279–1284
doi: 10.1248/bpb.33.1279
Brouwers EE, Huitema AD, Boogerd W, Beijnen JH, Schellens JH (2009) Persistent neuropathy after treatment with cisplatin and oxaliplatin. Acta Oncol 48(6):832–841
doi: 10.1080/02841860902806609
El Gharras H (2009) Polyphenols: food sources, properties and applications—a review. Int J Food Sci Technol 44(12):2512–2518
doi: 10.1111/j.1365-2621.2009.02077.x
Masuda T, Shimazawa M, Hara H (2017) Retinal diseases associated with oxidative stress and the effects of a free radical scavenger (Edaravone). Oxid Med Cell Longev 1–14
Boulikas T, Vougiouka M (2003) Cisplatin and platinum drugs at the molecular level (review). Oncol Rep 10(6):1663–1682
pubmed: 14534679
Borovskaya TG, Goldberg VE, Fomina TI, Pakhomova AV, Kseneva SI, Poluektova ME et al (2004) Morphological and functional state of rat ovaries in early and late periods after administration of platinum cytostatics. Bull Exp Biol Med 137:331–335
doi: 10.1023/B:BEBM.0000035121.85533.08
Dixit M, Yang JL, Poirier MC, Price JO, Andrews PA, Arteaga CL (1997) Abrogation of cisplatin induced programmed cell death in human breast cancer cells by epidermal growth factor antisense RNA. J Natl Cancer Inst 89(5):365–373
doi: 10.1093/jnci/89.5.365
Ourique GM, Saccol EM, Pês TS, Glanzner WG, Schiefelbein SH, Viviane M (2016) Protective effect of vitamin E on sperm motility and oxidative stress in valproic acid treated rats. Food Chem Toxicol 95:159–167
doi: 10.1016/j.fct.2016.07.011
Kara M, Daglioglu YK, Kuyucu Y, Tuli A, Tap O (2012) The effect of edaravone on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 162(2):197–202
doi: 10.1016/j.ejogrb.2012.02.026