Effect of A23187 ionophore treatment on human blastocyst development-a sibling oocyte study.
A23187 ionophore
Assisted oocyte activation
Blastocyst development
CultActive
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
04
10
2021
accepted:
15
03
2022
pubmed:
1
4
2022
medline:
10
6
2022
entrez:
31
3
2022
Statut:
ppublish
Résumé
To investigate whether treatment with commercially available ready-to-use A23187 ionophore (GM508-CultActive) improves embryo development outcome in patients with a history of embryo developmental problems. This is a uni-center prospective study in which sibling oocytes of patients with embryos of poor quality on day 5 in the previous cycle were treated or not with CultActive. Two hundred forty-seven metaphase II (MII) oocytes from 19 cycles performed between 2016 and 2019 were included in the study. After ICSI, the sibling oocytes were assigned to the treatment group or to the control group, following an electronically generated randomization list. A number of 122 MII were treated with CultActive and 125 MII had no treatment and were assigned to the control group. No difference in fertilization rate (p = 0.255) or in the capacity of embryos to reach good quality on day 5 (p = 0.197) was observed between the two groups. The utilization rates defined as the number of embryos transferred or cryopreserved per mature oocyte (p = 0.438) or per fertilized oocytes (p = 0.299) were not significantly different between the treated group and the control group. The results of the current study do not support the use of CultActive in cases with embryo developmental problems.
Identifiants
pubmed: 35357606
doi: 10.1007/s10815-022-02467-3
pii: 10.1007/s10815-022-02467-3
pmc: PMC9174380
doi:
Substances chimiques
Ionophores
0
Calcimycin
37H9VM9WZL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1225-1232Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Physiol Rev. 2006 Jan;86(1):25-88
pubmed: 16371595
Reproduction. 2004 Apr;127(4):431-9
pubmed: 15047934
J Assist Reprod Genet. 2018 Jun;35(6):1005-1010
pubmed: 29392515
J Assist Reprod Genet. 2015 Sep;32(9):1365-70
pubmed: 26292800
Cell Tissue Res. 2016 Jan;363(1):169-183
pubmed: 26453398
J Cell Physiol. 2006 Mar;206(3):565-73
pubmed: 16155907
Dev Biol. 2002 Oct 15;250(2):280-91
pubmed: 12376103
Dev Biol. 2006 Dec 15;300(2):534-44
pubmed: 16996050
Hum Reprod. 2015 Jan;30(1):97-102
pubmed: 25376461
Reprod Biomed Online. 2008 Nov;17(5):662-8
pubmed: 18983750
Reprod Biomed Online. 2006 Dec;13(6):791-4
pubmed: 17169196
Reproduction. 2006 Jan;131(1):27-34
pubmed: 16388006
Curr Top Dev Biol. 1995;30:21-62
pubmed: 7555047
Clin Exp Pharmacol Physiol. 2007 Oct;34(10):1084-9
pubmed: 17714098
Arch Gynecol Obstet. 2019 Oct;300(4):1083-1092
pubmed: 31529366
Reprod Biomed Online. 2020 Apr;40(4):501-509
pubmed: 32201115
Adv Biol Regul. 2013 Sep;53(3):292-308
pubmed: 23916605
Hum Reprod. 2009 Oct;24(10):2417-28
pubmed: 19584136
Hum Reprod Update. 2016 Jan-Feb;22(1):23-47
pubmed: 26346057
J Clin Invest. 2008 Nov;118(11):3671-81
pubmed: 18924610
J Assist Reprod Genet. 2021 Dec;38(12):3125-3133
pubmed: 34642877
Development. 2001 Mar;128(6):917-28
pubmed: 11222146
Reproduction. 2018 Feb;155(2):R105-R119
pubmed: 29122969
Fertil Steril. 2016 Sep 1;106(3):590-596.e2
pubmed: 27143515
Reprod Biomed Online. 2015 Dec;31(6):799-804
pubmed: 26507280
Development. 2002 Aug;129(15):3533-44
pubmed: 12117804