Barrier-Forming Potential of Epithelial Cells from the Exstrophic Bladder.
Journal
The American journal of pathology
ISSN: 1525-2191
Titre abrégé: Am J Pathol
Pays: United States
ID NLM: 0370502
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
23
12
2021
revised:
04
02
2022
accepted:
03
03
2022
pubmed:
1
4
2022
medline:
14
6
2022
entrez:
31
3
2022
Statut:
ppublish
Résumé
Bladder exstrophy (BEX) is a rare developmental abnormality resulting in an open, exposed bladder plate. Although normal bladder urothelium is a mitotically quiescent barrier epithelium, histologic studies of BEX epithelia report squamous and proliferative changes that can persist beyond surgical closure. The current study examined whether patient-derived BEX epithelial cells in vitro were capable of generating a barrier-forming epithelium under permissive conditions. Epithelial cells isolated from 11 BEX samples, classified histologically as transitional (n = 6) or squamous (n = 5), were propagated in vitro. In conditions conducive to differentiated tight barrier formation by normal human urothelial cell cultures, 8 of 11 BEX lines developed transepithelial electrical resistances of more than 1000 Ω.cm
Identifiants
pubmed: 35358476
pii: S0002-9440(22)00109-2
doi: 10.1016/j.ajpath.2022.03.009
pmc: PMC9194657
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
943-955Subventions
Organisme : Medical Research Council
ID : MR/N028414/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L013347/1
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Informations de copyright
Copyright © 2022 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
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