Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola.
Lysobacter antibioticus
Xanthomonas oryzae pv. oryzicola
biological control
phenazine substances
transcriptomic analysis
Journal
Journal of microbiology (Seoul, Korea)
ISSN: 1976-3794
Titre abrégé: J Microbiol
Pays: Korea (South)
ID NLM: 9703165
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
19
10
2021
accepted:
28
01
2022
revised:
24
01
2022
pubmed:
2
4
2022
medline:
4
5
2022
entrez:
1
4
2022
Statut:
ppublish
Résumé
Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most destructive diseases affecting rice production worldwide. In this study, we extracted and purified phenazine substances from the secondary metabolites of Lysobacter antibioticus 13-6. The bacteriostatic mechanism of phenazine substances against Xoc was investigated through physiological response and transcriptomic analysis. Results showed that phenazine substances affects the cell membrane permeability of Xoc, which causes cell swelling and deformation, blockage of flagellum synthesis, and imbalance of intracellular environment. The changes in intracellular environment affect the physiological and metabolic functions of Xoc, which reduces the formation of pathogenic factors and pathogenicity. Through transcriptomic analysis, we found that among differentially expressed genes, the expression of 595 genes was induced significantly (275 up-regulated and 320 down-regulated). In addition, we observed that phenazine substances affects three main functions of Xoc, i.e., transmembrane transporter activity, DNA-mediated transposition, and structural molecular activity. Phenazine substances also inhibits the potassium ion transport system that reduces Xoc resistance and induces the phosphate ion transport system to maintain the stability of the internal environment. Finally, we conclude that phenazine substances could retard cell growth and reduce the pathogenicity of Xoc by affecting cell structure and physiological metabolism. Altogether, our study highlights latest insights into the antibacterial mechanism of phenazine substances against Xoc and provides basic guidance to manage the incidence of bacterial leaf streak of rice.
Identifiants
pubmed: 35362894
doi: 10.1007/s12275-022-1542-0
pii: 10.1007/s12275-022-1542-0
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Phenazines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
496-510Informations de copyright
© 2022. The Microbiological Society of Korea.
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