Functional redundancy among Polycomb complexes in maintaining the pluripotent state of embryonic stem cells.

Cell Differentiation / genetics Drosophila Proteins / metabolism Embryonic Stem Cells / metabolism Polycomb Repressive Complex 1 / genetics Polycomb Repressive Complex 2 / genetics Polycomb-Group Proteins / genetics
PCGF PRC1 Polycomb RING1A/B cPRC1 embryonic stem cells germ layer lineages ncPRC1 pluripotency redundancy

Journal

Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300

Informations de publication

Date de publication:
10 05 2022
Historique:
received: 22 09 2021
revised: 27 02 2022
accepted: 28 02 2022
pubmed: 2 4 2022
medline: 18 5 2022
entrez: 1 4 2022
Statut: ppublish

Résumé

Polycomb group proteins assemble into multi-protein complexes, known as Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), that guide cell fate decisions during embryonic development. PRC1 forms an array of biochemically distinct canonical PRC1 (cPRC1) or non-canonical PRC1 (ncPRC1) complexes characterized by the mutually exclusive presence of PCGF (PCGF1-PCGF6) paralog subunit; however, whether each one of these subcomplexes fulfills a distinct role remains largely controversial. Here, by performing a CRISPR-based loss-of-function screen in embryonic stem cells (ESCs), we uncovered a previously unappreciated functional redundancy among PRC1 subcomplexes. Disruption of ncPRC1, but not cPRC1, displayed severe defects in ESC pluripotency. Remarkably, coablation of non-canonical and canonical PRC1 in ESCs resulted in exacerbation of the phenotype observed in the non-canonical PRC1-null ESCs, highlighting the importance of functional redundancy among PRC1 subcomplexes. Together, our studies demonstrate that PRC1 subcomplexes act redundantly to silence lineage-specific genes and ensure robust maintenance of ESC identity.

Identifiants

DOI: 10.1016/j.stemcr.2022.02.020 PMID: 35364009 PMC: PMC9120860
pubmed: 35364009
pii: S2213-6711(22)00133-3
doi: 10.1016/j.stemcr.2022.02.020
pmc: PMC9120860
pii:
doi:

Substances chimiques

Drosophila Proteins 0
Polycomb-Group Proteins 0
Polycomb Repressive Complex 2 EC 2.1.1.43
Polycomb Repressive Complex 1 EC 2.3.2.27

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1198-1214

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Yaru Zhu (Y)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Lixia Dong (L)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Congcong Wang (C)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Kunying Hao (K)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Jingnan Wang (J)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Linchun Zhao (L)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Lijun Xu (L)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China.

Yin Xia (Y)

School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.

Qing Jiang (Q)

Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing, China.

Jinzhong Qin (J)

State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Medical School of Nanjing University, 12 Xuefu Road, Nanjing, Jiangsu 210061, China; Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing 210093, China. Electronic address: qinjz@nju.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Functional redundancy among Polycomb complexes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Functional redundancy among Polycomb complexes...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Functional redundancy among Polycomb complexes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Protéines du groupe Polycomb › Complexe répresseur Polycomb-1 › Functional redundancy among Polycomb complexes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Protéines du groupe Polycomb › Complexe répresseur Polycomb-2 › Functional redundancy among Polycomb complexes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines de répression › Protéines du groupe Polycomb › Functional redundancy among Polycomb complexes...