Donor T cells for CAR T cell therapy.

CRISPR-Cas9 Donor CAR T cells GVHD Genome editing TALENs

Journal

Biomarker research
ISSN: 2050-7771
Titre abrégé: Biomark Res
Pays: England
ID NLM: 101607860

Informations de publication

Date de publication:
01 Apr 2022
Historique:
received: 13 12 2021
accepted: 26 02 2022
entrez: 2 4 2022
pubmed: 3 4 2022
medline: 3 4 2022
Statut: epublish

Résumé

Adoptive cell therapy using patient-derived chimeric receptor antigen (CAR) T cells redirected against tumor cells has shown remarkable success in treating hematologic cancers. However, wider accessibility of cellular therapies for all patients is needed. Manufacture of patient-derived CAR T cells is limited by prolonged lymphopenia in heavily pre-treated patients and risk of contamination with tumor cells when isolating T cells from patient blood rich in malignant blasts. Donor T cells provide a good source of immune cells for adoptive immunotherapy and can be used to generate universal off-the-shelf CAR T cells that are readily available for administration into patients as required. Genome editing tools such as TALENs and CRISPR-Cas9 and non-gene editing methods such as short hairpin RNA and blockade of protein expression are currently used to enhance CAR T cell safety and efficacy by abrogating non-specific toxicity in the form of graft versus host disease (GVHD) and preventing CAR T cell rejection by the host.

Identifiants

pubmed: 35365224
doi: 10.1186/s40364-022-00359-3
pii: 10.1186/s40364-022-00359-3
pmc: PMC8973942
doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

14

Subventions

Organisme : Inner Wheel Foundation
ID : Cord blood: a new cell source for T cell engineering
Organisme : Inner Wheel Foundation
ID : adoptive immunotherapy

Informations de copyright

© 2022. The Author(s).

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Auteurs

Tiffany C Y Tang (TCY)

Graduate School of Biomedical Engineering, Faculty of Engineering, UNSW Sydney, Sydney, NSW, Australia. tiffany.tang@unsw.edu.au.
Children's Cancer Institute, Lowy Cancer Research Center, UNSW Sydney, Sydney, NSW, Australia. tiffany.tang@unsw.edu.au.

Ning Xu (N)

Children's Cancer Institute, Lowy Cancer Research Center, UNSW Sydney, Sydney, NSW, Australia.
School of Women's and Children's Health, Faculty of Medicine, UNSW Sydney, Sydney, NSW, Australia.

Robert Nordon (R)

Graduate School of Biomedical Engineering, Faculty of Engineering, UNSW Sydney, Sydney, NSW, Australia.

Michelle Haber (M)

Children's Cancer Institute, Lowy Cancer Research Center, UNSW Sydney, Sydney, NSW, Australia.
School of Women's and Children's Health, Faculty of Medicine, UNSW Sydney, Sydney, NSW, Australia.
Kids Cancer Center, Sydney Children's Hospital, Sydney, NSW, Australia.

Kenneth Micklethwaite (K)

Blood Transplant and Cell Therapies Program, Department of Hematology, Westmead Hospital, Sydney, NSW, Australia.
Blood Transplant and Cell Therapies Laboratory, NSW Health Pathology, ICPMR Westmead, Sydney, NSW, Australia.
Westmead Institute for Medical Research, Sydney, NSW, Australia.
Sydney Medical School, The University of Sydney, Sydney, NSW, Australia.

Alla Dolnikov (A)

Children's Cancer Institute, Lowy Cancer Research Center, UNSW Sydney, Sydney, NSW, Australia.
School of Women's and Children's Health, Faculty of Medicine, UNSW Sydney, Sydney, NSW, Australia.
Kids Cancer Center, Sydney Children's Hospital, Sydney, NSW, Australia.

Classifications MeSH