Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d'Ivoire.
Asymptomatic
Côte d’Ivoire
Korhogo
LAMP
Microscopy
PCR
Plasmodium spp.
RDT
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
02 Apr 2022
02 Apr 2022
Historique:
received:
09
07
2021
accepted:
21
03
2022
entrez:
3
4
2022
pubmed:
4
4
2022
medline:
6
4
2022
Statut:
epublish
Résumé
Highly sensitive and accurate malaria diagnostic tools are essential to identify asymptomatic low parasitaemia infections. This study evaluated the performance of histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (RDTs), microscopy and loop-mediated isothermal amplification (LAMP) for the detection of asymptomatic Plasmodium spp. infections in Northern Côte d'Ivoire, using nested polymerase chain reaction (nPCR) as reference. A household-based survey was carried out in July 2016, in the health district of Korhogo, involving 1011 adults without malaria symptom nor history of fever during the week before recruitment. The fresh capillary blood samples were collected to detect Plasmodium infections using on HRP-2-based RDTs, microscopy and LAMP and stored as dried blood spots (DBS). A subset of the DBS (247/1011, 24.4%) was randomly selected for nPCR analyses. Additionally, venous blood samples, according to LAMP result (45 LAMP positive and 65 LAMP negative) were collected among the included participants to perform the nested PCR used as the reference. The prevalence of asymptomatic Plasmodium spp. infections determined by RDT, microscopy, and LAMP were 4% (95% confidence interval (CI) 2.8-5.3), 5.2% (95% CI 3.9-6.6) and 18.8% (95% CI 16.4-21.2), respectively. Considering PCR on venous blood as reference, performed on 110 samples, the sensibility and specificity were, respectively, 17.8% (95% CI 6.1-29.4) and 100% for RDT, 20.0% (95% CI 7.8-32) and 100% for microscopy, and 93.3% (95% CI 85.7-100) and 95.4% (95% CI 92.2-100) for LAMP. In Northern Côte d'Ivoire, asymptomatic Plasmodium infection was found to be widely distributed as approximately one out of five study participants was found to be Plasmodium infected. LAMP appears currently to be the only available diagnostic method that can identify in the field this reservoir of infections and should be the method to consider for potential future active case detection interventions targeting elimination of these infections.
Sections du résumé
BACKGROUND
BACKGROUND
Highly sensitive and accurate malaria diagnostic tools are essential to identify asymptomatic low parasitaemia infections. This study evaluated the performance of histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (RDTs), microscopy and loop-mediated isothermal amplification (LAMP) for the detection of asymptomatic Plasmodium spp. infections in Northern Côte d'Ivoire, using nested polymerase chain reaction (nPCR) as reference.
METHODS
METHODS
A household-based survey was carried out in July 2016, in the health district of Korhogo, involving 1011 adults without malaria symptom nor history of fever during the week before recruitment. The fresh capillary blood samples were collected to detect Plasmodium infections using on HRP-2-based RDTs, microscopy and LAMP and stored as dried blood spots (DBS). A subset of the DBS (247/1011, 24.4%) was randomly selected for nPCR analyses. Additionally, venous blood samples, according to LAMP result (45 LAMP positive and 65 LAMP negative) were collected among the included participants to perform the nested PCR used as the reference.
RESULTS
RESULTS
The prevalence of asymptomatic Plasmodium spp. infections determined by RDT, microscopy, and LAMP were 4% (95% confidence interval (CI) 2.8-5.3), 5.2% (95% CI 3.9-6.6) and 18.8% (95% CI 16.4-21.2), respectively. Considering PCR on venous blood as reference, performed on 110 samples, the sensibility and specificity were, respectively, 17.8% (95% CI 6.1-29.4) and 100% for RDT, 20.0% (95% CI 7.8-32) and 100% for microscopy, and 93.3% (95% CI 85.7-100) and 95.4% (95% CI 92.2-100) for LAMP.
CONCLUSION
CONCLUSIONS
In Northern Côte d'Ivoire, asymptomatic Plasmodium infection was found to be widely distributed as approximately one out of five study participants was found to be Plasmodium infected. LAMP appears currently to be the only available diagnostic method that can identify in the field this reservoir of infections and should be the method to consider for potential future active case detection interventions targeting elimination of these infections.
Identifiants
pubmed: 35366883
doi: 10.1186/s12936-022-04133-6
pii: 10.1186/s12936-022-04133-6
pmc: PMC8976314
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111Informations de copyright
© 2022. The Author(s).
Références
PLoS One. 2017 Oct 5;12(10):e0185742
pubmed: 28982155
Lancet Infect Dis. 2018 Oct;18(10):1108-1116
pubmed: 30170986
Trans R Soc Trop Med Hyg. 2014 Aug;108(8):488-94
pubmed: 24907711
Malar J. 2012 Dec 06;11:408
pubmed: 23217163
Malar J. 2015 Dec 21;14:509
pubmed: 26692284
Expert Rev Mol Diagn. 2018 Feb;18(2):195-203
pubmed: 29353522
PLoS One. 2018 Jun 13;13(6):e0182304
pubmed: 29897901
Nature. 2015 Dec 3;528(7580):S86-93
pubmed: 26633770
Infect Dis Poverty. 2018 Apr 25;7(1):39
pubmed: 29690913
PLoS One. 2013 Dec 16;8(12):e82387
pubmed: 24358177
Malar J. 2014 Apr 07;13:137
pubmed: 24708551
J Infect Dis. 2013 Aug 15;208(4):645-52
pubmed: 23633405
Biochem Med (Zagreb). 2012;22(3):276-82
pubmed: 23092060
PLoS Med. 2013;10(6):e1001467
pubmed: 23853551
PLoS One. 2017 Jan 17;12(1):e0169037
pubmed: 28095434
PLoS One. 2015 Nov 10;10(11):e0142842
pubmed: 26555068
J Infect Dis. 2013 Aug 15;208(4):637-44
pubmed: 23633403
Malar J. 2015 Jan 05;14:7
pubmed: 25559587
Methods Mol Med. 2002;72:189-203
pubmed: 12125116
Malar J. 2015 Feb 25;14:95
pubmed: 25849211
Malar J. 2015 Jan 28;14:43
pubmed: 25627037
J Infect Dis. 2014 Oct 15;210(8):1167-9
pubmed: 24795471
PLoS One. 2014 Aug 08;9(8):e103905
pubmed: 25105591
Malar J. 2015 May 17;14:205
pubmed: 25982190
Malar J. 2015 Jan 28;14:44
pubmed: 25626339
Malar J. 2015 Oct 09;14:396
pubmed: 26450599
Nat Rev Microbiol. 2014 Dec;12(12):833-40
pubmed: 25329408